母爱剥夺对子代鼠前额叶皮质c-fos蛋白表达的影响  

Effect of Early Maternal Deprivation on Expression of c-fos in Prefrontal Cortex of Offspring Rats

作　　者：邝满元[1] 邓鹏程[1] 徐松[1] 

机构地区：[1]湘南学院解剖教研室,湖南郴州423000

出　　处：《解剖科学进展》2009年第2期199-201,共3页Progress of Anatomical Sciences

基　　金：郴州市科技局立项资助项目(2008gl05)

摘　　要：目的探讨母爱剥夺对子代鼠前额叶皮质c-fos蛋白表达的影响。方法将20只刚出生的仔鼠称重后,随机分为母爱剥夺组(MD)和对照组(N),母爱剥夺组仔鼠与母鼠每天间断隔离4h,对照组母鼠和仔鼠不隔离。第9d、21d称重后处死。用免疫组织化学方法(ABC法)检测前额叶皮质c-fos的表达,图像分析系统测定阳性反应产物的平均灰度值。结果两组仔鼠出生时体重无明显差别,在9d和21d时,母爱剥夺组仔鼠体重显著低于对照组仔鼠(P<0.05)。母爱剥夺9天和21天与相应对照组相比,前额叶皮质c-fos的表达均显著降低(P<0.05)。结论母爱剥夺影响子代鼠生长和c-fos表达。Objective To investigate the effects of early maternal deprivation on the expression of c-los in prefrontal cortex of offspring rats. Methods 20 rats born at the same day were weighed and divided into maternal deprivation group and control groups averagely, the rats of maternal deprivation group were rerepeatedly seperated from their mother for periods of up to 4h per day, without seperation for the control rats. Then the rats were sacrificed at the 9d,21d after weighing. The expression of c-los in prefrontal cortex was observed by immunohistochemical technique （ABC method）, and the mean gray values of immunoreactive product were analyzed by image analysis system. Results There were no differences on birth weight between the two groups, but the weight decreased significantly in maternal deprivation group than in control group （ P 〈 0. 05） at 9d and 21d, with lower expression of c-fos in maternal deprivation group compared with control group （ P 〈 0. 05）. Conclusion Deprivation of mother loving can cause the decrease of the weight of offspring rats and downregulation of c-fos expression in prefrontal cortex.

关 键 词：母爱剥夺 c—fos 前额叶皮质 子代鼠 

分 类 号：R338.25[医药卫生—人体生理学]