Proteome changes in the plasma of Papilio xuthus (Lepidoptera:Papilionidae):effect of parasitization by the endoparasitic wasp Pteromalus puparum (Hymenoptera:Pteromalidae)  

作　　者：Jia-ying ZHU Gong-yin YE Qi FANG Cui HU 

机构地区：[1]State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2009年第6期445-453,共9页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project supported by the National Basic Research Program (973) of China (No. 2006CB102005);the National Natural Science Foundation of China (Nos. 30571251 and 30170626);the Program for New Cen-tury Excellent Talents in University of the Ministry of Education of China (No. NCET-05-0513);the Innovation Research Team Program of the Ministry of Education of China (No. IRT0535)

摘　　要：Although the biochemical dissection of parasitoid-host interactions is becoming well characterized, the molecular knowledge concerning them is minimal. In order to understand the molecular bases of the host immune response to parasitoid attack, we explored the response of Papilio xuthus parasitized by the endoparasitic wasp Pteromalus puparum using proteomic approach. By examining the differential expression of plasma proteins in the parasitized and unparasitized host pupae by two-dimensional (2D) electrophoresis, 16 proteins were found to vary in relation to parasitization compared with unparasitized control samples. All of them were submitted to identification by mass spectrometry coupled with a database search. The modulated proteins were found to fall into the following functional groups: humoral or cellular immunity, detoxification, energy metabolism, and others. This study contributes insights into the molecular mechanism of the relationships between parasitoids and their host insects.Although the biochemical dissection of parasitoid-host interactions is becoming well characterized, the molecular knowledge concerning them is minimal. In order to understand the molecular bases of the host immune response to parasitoid attack, we explored the response of Papilio xuthus parasitized by the endoparasitic wasp Pteromalus puparum using proteomic approach. By examining the differential expression of plasma proteins in the parasitized and unparasitized host pupae by two-dimensional （2D） electrophoresis, 16 proteins were found to vary in relation to parasitization compared with unparasitized control samples. All of them were submitted to identification by mass spectrometry coupled with a database search. The modulated proteins were found to fall into the following functional groups： humoral or cellular immunity, detoxification, energy metabolism, and others. This study contributes insights into the molecular mechanism of the relationships between parasitoids and their host insects.

关 键 词：Proteomics PLASMA PARASITISM Immune response Pteromaluspuparum Papilio xuthus 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治] Q51[农业科学—植物保护]