山荷减肥颗粒对饮食诱导肥胖大鼠瘦素、脂联素、抵抗素的影响  

Effects of Shan He Jian Fei Granule on the expression of leptin,adiponectin and resistin in obese rats induced by high-fat feeding

作　　者：孙升云[1] 黄进[1,2] 孟民杰[3] 陆家海[4] 杨钦河[1] 张荣华[1] 朱晓峰[1] 刘康丽[1] 

机构地区：[1]暨南大学附属第一医院中西医结合气血研究室,广东广州510630 [2]广州中医药大学 [3]广东药学院生命科学与生物制药学院,广东广州510006 [4]中山大学公共卫生学院,广东广州510080

出　　处：《中国病理生理杂志》2009年第6期1170-1174,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30271635);广东省自然科学基金资助项目(No.7005956)

摘　　要：目的:观察山荷减肥颗粒对高脂饮食诱导的肥胖模型大鼠的减肥降脂作用及对脂肪组织脂联素、抵抗素基因表达的影响。方法:肥胖模型大鼠随机分为山荷减肥颗粒高、中、低剂量3个实验组及高脂对照组、正常组。对给药8周的动物称体重,测体长,计算Lee’s指数,测定大鼠血清胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)及高密度脂蛋白(HDL)的水平,用ELISA法测瘦素水平,用RT-PCR检测附睾周围脂肪组织脂联素、抵抗素mRNA表达。结果:山荷减肥颗粒组大鼠体重、Lee’s指数、脂肪重量、血清TC、TG、LDL水平明显低于高脂组大鼠(P<0.05),瘦素明显低于高脂模型组(P>0.05),脂肪组织脂联素、抵抗素mRNA的表达水平明显高于高脂组大鼠(P<0.05)。结论:山荷减肥颗粒对肥胖大鼠具有减肥降脂作用,调整肥胖大鼠脂肪组织瘦素、脂联素、抵抗素mRNA的表达水平,可能是其治疗肥胖的作用机制之一。AIM ： To study the effects of Shan He Jian Fei Granule （ SHJFG）, a Chinese medicine, on weight - reduction and fat - decrease in adiposity rats, and to observe the changes of leptin, adiponectin and resistin. METHODS： After the models were prepared successfully, the rats were randomly divided into four groups： normal group, low dosage, middle dosage, high dosage and obesity control group. After 8 weeks interference with SHJFG, the weight and the naso - anal length of each rat was measured and Lee＇s index were calculated. The levels of TG, TC, LDL, HDL and leptin in serum were carefully determined. The gene expressions of adiponectin and resistin in adipose tissues of rats were deter- mined by RT - PCR. RESULTS ： Compared to high - fat diet group, the body weights, the Lee＇s indexes, the weight of fat tissues and levels of TG, TC, LDL and leptin in SHJFG groups significantly decreased （ P 〈 0. 05 ）. Adiponectin mRNA expression in SHJFG group significantly increased （ P 〈 0. 05 ）, and the resistin mRNA expression also significantly increased. CONCLUSION： SHJFG significantly decreases the body weight and the serum levels of TG, TC and LDL in obese rats. The effects of SHJFG in raising the mRNA expressions of adiponectin and resistin in fat tissues may be one of the main role that results in lowering body weight in obese rats.

关 键 词：肥胖症 高脂饮食 山荷减肥颗粒 脂联素 抵抗素 瘦素 

分 类 号：R255.8[医药卫生—中医内科学]