低氧及其模拟物CoCl2下调Foxp3的表达不依赖于HIF-1α  

Hypoxia and Its Simulant CoCl_2 Down-regulates Foxp3 Expression Independent from HIF-1α

作　　者：王晓东 刘传绪[2] 吴英理[2] 陈静[1] 

机构地区：[1]上海交通大学医学院附属上海儿童医学中心血液肿瘤科,上海200127 [2]上海交通大学医学院病理生理教研室,上海200025

出　　处：《中国实验血液学杂志》2009年第3期533-536,共4页Journal of Experimental Hematology

摘　　要：本研究探讨低氧条件下人T-淋巴细胞白血病Jurkat细胞株Foxp3表达水平及在该过程中HIF-1α的作用,并研究低氧影响调节性T细胞功能的作用机制。应用低氧(1%CO2)及其模拟化合物CoCl2处理Jurkat细胞并在不同时相后用Western blot检测HIF-1表达情况,同时应用real-time PCR检测Foxp3表达水平;应用RNA干扰技术抑制Jurkat细胞HIF-1α基因表达,然后分别检测HIF-1α及Foxp3的表达水平。结果表明:Jurkat细胞经过低氧(1%CO2)及其模拟化合物CoCl2处理后,HIF-1α有明显累积,而Foxp3表达明显下降;Jurkat细胞的HIF-1基因经siRNA干扰抑制后,低氧模拟化合物CoCl2依然可以下调Foxp3表达。结论:低氧及其模拟物CoCl2可以明显下调Foxp3表达,但该过程不依赖于HIF-1。This study was purposed to investigate the expression of Jurkat cell Foxp3 in hypoxia condition and the role of HIF-1α in this process as well as to clarify the mechanism influencing function of regulatory T cells by hypoxia. The Jurkat ceils were incubated with hypoxia （1% O2 ） and its simulant COCl2 for different times （0,6,12,24 hours）, the viability was measured by trypan blue staining, the expression of HIF-1α was detected by Western blot, the expression of Foxp3 was detected by real-time PCR, the expressions of HIF-1α and Foxp3 were assayed after HIF-1α in Jurkat cells was inhibited by using RNA interference technique. The results indicated that after Jurkat ceils were treated with hy- poxia and its simulant CoCl2 , the significant accumulation of HIF-1α in cells appeared, but the expression of Foxp3 was obviously down-regulated; after expression of HIF-1α in Jurkat cells was inhibited by siRNA interference, the CoCl2 still could down-regulate the expression of Foxp3. It is concluded that the hypoxia and its simulant CoCl2 can obviously down-regulate the expression of Foxp3, but this process is independent from HIF-1 α.

关 键 词：低氧 FOXP3 调节T细胞 HIF—1α JURKAT细胞 

分 类 号：O613.3[理学—无机化学] R733.1[理学—化学]