微流体芯片技术在ABO血型基因分型中的应用  

Application of Microfluidic Chip Analytical Systems in ABO Genotyping

作　　者：刘长利[1] 龚晓燕[1] 王卓妍[1] 任芙蓉[1] 吕秋霜[1] 苗天红[1] 戴苏娜[1] 

机构地区：[1]北京市红十字血液中心,北京100088

出　　处：《中国实验血液学杂志》2009年第3期793-796,共4页Journal of Experimental Hematology

摘　　要：本研究建立一种简单、可靠的ABO基因检测结果报告评估方法。利用微流体芯片检测技术代替凝胶电泳,通过对实验条件的优化,进行multiplex-PCR-RFLP的ABO基因分型结果判读,并通过对150份标本进行测试,对该检测方法的稳定性和准确性进行评估。结果显示,全部检测结果与血清学分析结果一致,并且检测出1例因肿瘤引起的B型抗原减弱病例。结论:建立的微流芯片基因检测系统稳定、可靠,实现了ABO基因分型检测结果的客观化、标准化和自动化。Limitations of polyacrylamide gel or agarose gel electrophorefic methods in genotyping research affect the interpreting of detection results. In order to develop a simple and reliable method for appraising results of ABO genotyping detection, the microfluidic chip analysis system was established by using microfluidic chip to replace the gel electrophoresis and combining with multiplex-PCR-RFLP technique. 150 blood samples were tested by this microfluidic chip analysis system with multiplex-PCR-RFLP technique to evaluate its stability and accuracy. The results showed that all the testing results were consistent with serologic ABO genotyping results and 1 blood sample with decrease of B antigen caused by CML was identified. In conclusion, the established microfluidic chip analysis system is stable and reliable technique. Application of this technique enables the ABO genotyping results to be more objective and accurate.

关 键 词：ABO基因分型 微流体芯片 应用研究 

分 类 号：R446.11[医药卫生—诊断学] R457.11[医药卫生—临床医学]