双氢青蒿素诱导前列腺癌PC-3细胞凋亡的形态学研究  

Morphological study on dihydroartemisinin in inducing apoptosis of human prostate cancer cell line PC-3

作　　者：廖光东[1] 李庆春[1] 高小玲[1] 罗子国[1] 

机构地区：[1]重庆医科大学生命科学研究院,重庆400016

出　　处：《激光杂志》2009年第3期88-89,共2页Laser Journal

摘　　要：目的:研究双氢青蒿素(DHA)诱导前列腺癌PC-3细胞凋亡作用,并观察其形态学变化。方法:以前列腺癌PC-3细胞为研究对象,在体外经不同浓度的DHA处理48小时后,扫描电镜(SEM)观察细胞凋亡;并建立PC-3细胞裸鼠种植瘤模型,20只模型鼠随机分为对照组、溶剂组、DHA大剂量组及DHA小剂量组,经13天干预后,透射电镜(TEM)观察肿瘤组织形态学变化,体视学分析肿瘤微血管密度(MVD)。结果:PC-3细胞经DHA处理后,SEM观察到典型的凋亡细胞;裸鼠种植瘤经治疗后,TEM观察肿瘤组织中散在凋亡细胞及凋亡小体,且DHA大小剂量组MVD均低于对照组(P<0.05)。结论:DHA具有较强的抗肿瘤作用,其作用机制可能与诱导肿瘤细胞凋亡有关。Objective：To study the effects of dihydroartemisinin （DHA） on inducing apoptasis of human prostate cancer PC- 3 cells and investigate the morphological changes. Methods： As the study object, human prostate cancer cell line PC - 3 cells were treated by dihydroartemisinin of different concentration for 48 hours in vitro. Then PC- 3 cells were scanned by scanning electron microscope （SEM） .And prostate cancer PC - 3 cells were transplanted into 20 nude mice to establish the solid tumor mode. These nude mice were randomly divided into 4 groups with 5 mice in each group： control group, solvent group, large dose DHA group and low dose DHA group. Histomorphology changes of PC - 3 cells were observed by transmission electron micmoscope（TEM） after administration of 13 days. Micro-vasvulor density（MVD） was analysed by stereological method. Results： The apoptotic cell were observed by SEM after treatment of DHA. TEM examination revealed that the scattered apoptotic cells and apoptotic bodies were observed in tumor tissues of DHA groups after administration of drugs;And MVD was significantly decreased in DHA groups（p 〈 0.05）. Conclusion： These results demonstrated that DHA had stronger effect of inducing apoptosis on human prostate cancer cell line PC - 3 cells in vitro and vivo. The action mechanism might be related with DHA inducing the apoptesis of prostate cancer PC - 3 cell.

关 键 词：双氢青蒿素 前列腺癌 凋亡 微血管密度 

分 类 号：R284.1[医药卫生—中药学] R979.1[医药卫生—中医学]