栀子苷对氧化应激损伤血管内皮细胞的保护作用  

Protective effects of geniposide on human umbilical vein endothelial cell injury induced by H_2O_2 in vitro

作　　者：丁嵩涛[1] 刘洪涛[2] 李文明[1] 李秀英[1] 于超[1] 

机构地区：[1]重庆医科大学生命科学研究院,重庆400016 [2]中国科学院大连化学物理研究所,辽宁大连116023

出　　处：《中国药理学通报》2009年第6期725-729,共5页Chinese Pharmacological Bulletin

基　　金：重庆自然科学基金资助项目(NoKJ070304);重庆医科大学人才启动基金资助项目(No0200100263)

摘　　要：目的研究栀子苷对体外培养的人脐静脉内皮细胞(HUVEC)氧化损伤的保护作用。方法采用过氧化氢(Hy-drogen peroxide,H2O2)建立体外培养的HUVEC细胞氧化应激损伤模型。将细胞分为正常对照组、H2O2氧化损伤组、H2O2加栀子苷低、中、高剂量组,其中后3组给予栀子苷预培养24h后加入400μmol.L-1H2O2,然后继续培养12h。MTT法检测细胞存活率;检测各组细胞内超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、一氧化氮合酶(NOS)水平和培养液中一氧化氮(NO)水平;检测细胞内活性氧簇(ROS)水平;用流式细胞仪检测细胞凋亡及周期改变。结果栀子苷能明显提高H2O2损伤的内皮细胞的存活率,提高细胞内SOD、GSH-Px、NOS活性,使培养液中NO含量增加,降低细胞内ROS水平,减少H2O2诱导的细胞凋亡率,恢复血管内皮细胞增殖。结论栀子苷具有较强的抗氧化能力及内皮细胞保护作用,可减轻血管内皮细胞的氧化损伤。Aim To study the protective effect and mechanism of geniposide on human umbilical vein endothelial cell （HUVEC） injury induced by H2O2. Methods The injured model was established by HUVEC treated with H2O2. HUVECs were cultured in vitro and divided into five groups： control group, H2O2 group and geniposide with different concentrations plus H2O2 group respectively. HUVECs were incubated with 400μmol.L^-1 H2O2 for 12 hours in the absence or presence of various concentrations of geniposide pre-incubation. Survival rate of HUVECs was determined by tetrazolium assay. The intracellular activities of superoxide dismutase （SOD） , glutathione peroxidase （ GSHPx）, total nitric oxide synthase（NOS） and extracellular nitric oxide （NO） level were detected. The intracellular reactive oxygen species （ROS） level and the apoptotic index and cell cycle alteration were detected by flow cytometry. Results Geniposide concentration-dependently increased the viability of injured endotheial cells, while increased the activity of SOD, GSH-Px, NOS and NO production. The intracellular ROS level and the apoptotic index were reduced by geniposide. The cell proliferation was increased with geniposide incubation. Conclusion Geniposid may be a potential anti-oxidation agent which has a protective effect against HUVEC injuries induced by H2O2.

关 键 词：栀子苷 氧化应激损伤 人脐静脉内皮细胞 细胞凋亡 

分 类 号：R284.1[医药卫生—中药学] R322.12[医药卫生—中医学]