肉苁蓉多糖的巨噬细胞活化作用  

The effect of Cistanche deserticola polysaccharides(CDPS) on marcrophages activation

作　　者：王翔岩[1] 齐云[1] 蔡润兰[1] 李晓红[1] 杨美华[1] 石钺[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《中国药理学通报》2009年第6期787-790,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30672659);宁夏自治区科技攻关计划项目(No05GG-10807)

摘　　要：目的观察肉苁蓉多糖(CDPS)对巨噬细胞的活化作用。方法采用中性红法测定吞噬活性;Griess试剂法测定NO释放量;L929细胞法及小鼠胸腺细胞法测定TNF-α及IL-1释放。结果CDPS在6.25～50mg.L-1剂量范围内可剂量依赖性地增强BALB/c小鼠腹腔巨噬细胞吞噬中性红能力和促进NO释放;在2.8～100mg.L-1剂量范围内可使正常和免疫抑制的RAW264.7细胞NO释放明显增加;在0.56～7.2mg.L-1或3.6～10mg.L-1范围促进RAW264.7细胞TNF-α或IL-1产生,均具有良好的量效关系。结论CDPS可明显提高巨噬细胞吞噬及分泌功能,活化巨噬细胞。肉苁蓉免疫增强作用可能与此有关。Aim To investigate the activation effect of Cistanche deserticola polysaccharides （ CDPS ） on marcrophages. Methods The phagocytic ability of peritoneal macrophage （MФ） was evaluated with neutral red dye phagocytosis assay;nitric oxide （NO） production was examined by Griess reaction;the activity of tumor necrosis factor-α （TNF-α） and interleukin-1 （ IL- 1 ） secreted were measured by L929 cells bioassay and proliferation of mouse thymocyte. Results CDPS at the concentration range of 6. 25 -50 mg.L^-1 enhanced the phagocytic ability and NO release of peritoneal macrophage in a dose-dependent manner. CDPS at the dose of 2.8 -100 mg . L^-1 significantly increased NO secretion of normal or immunesuppressed RAW264.7 cells. TNF-α production by RAW264. 7 cells was promoted after incubation of RAW264.7 cells with CDPS at the concentration of 0. 56 - 7.2 mg.L^-1. IL-1 release of RAW264. 7 eells was obviously elevated by CDPS at the dose of 3.6 - 10 mg.L^-1 , and they both showed a good dose-effeet relationship. Conclusions CDPS can markedly promote the phagocytic and secretory functions of MФ, and activate MФ, which suggests that the immunomodulatory effect of CDPS may be related to it.

关 键 词：肉苁蓉多糖 巨噬细胞 吞噬活性 NO TNF—α IL-1 

分 类 号：R332[医药卫生—人体生理学] R284.1[医药卫生—基础医学]