人成纤维样滑膜细胞冻存时间对其活性的影响  

Influence of cryopreservation on the survival of fibroblast-like synoviocytes

作　　者：戴杏[1] 郭晓蓉[1] 常艳[1] 魏伟[1] 

机构地区：[1]安徽医科大学临床药理研究所,抗炎免疫药理学省部共建教育部重点实验室,抗炎免疫药物安徽省工程技术研究中心,安徽合肥230032

出　　处：《中国药理学通报》2009年第6期828-830,共3页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30572356);安徽省科技攻关计划项目(No06013133B);高等学校博士学科点专项科研基金(No20060366003);高校首批科技创新团队基金(No2005TD003);安徽省研究实验基地优秀中青年科研带头人基金(No200622805);安徽医科大学校科研基金(No2008kj13)

摘　　要：目的探讨适合人成纤维样滑膜细胞(FLS)低温冷藏保存的最佳冻存时间。方法将FLS经-80℃冰箱冷冻保存。在冷冻后2、3、4、5wk分别复苏,培养。观察FLS的生长情况和细胞存活率及24h贴壁率。结果冻存5wk的FLS细胞存活率(66±1.3)%,较冻存2wk(90±2.0)%有明显下降;冻存5wk的FLS24h贴壁率(61.3±2)%,与冻存2wk(85.9±3)%比较有明显下降;未冻存和冻存2wk复苏后FLS的增殖能力无明显差异。结论FLS可以进行低温冷冻,冻存时间4wk为宜。Aim To determine appropriate length of cryopreservation time for fibroblast-like synoviocytes. Methods FLS were cryopreserved in - 80℃ refrigerator, resuscitated and cultured after being frozed for 2,3,4 and 5 weeks. The cell viability and 24 hours adherence rate were evaluated. Results There was significant difference in the rate of cell livability of FLS cryopreserved for 5 weeks compared with that for 2 weeks. Significant difference was also observed in the 24 hours adherence rate of FLS cryopreserved for 5 weeks compared with that for 2 weeks. There was no difference in the proliferation of FLS routinely maintained compared with those cryopreserved for 2 weeks. Conclusions FLS can be cryopreserved and the time limit of cryopreservation should be 4 weeks.

关 键 词：人成纤维样滑膜细胞 冻存时间 活性 

分 类 号：R318.52[医药卫生—生物医学工程] R329.2[医药卫生—基础医学]