爆炸冲击波致肝组织超微结构改变  

Explosive blast-induced ultrastructural changes of goat liver tissues

作　　者：马晓艳[1,2] 黄晓峰[1] 任东青[3] 尹文[1] 王文勇[4] 王爽[1] 李勇强[1] 于华[1] 

机构地区：[1]第四军医大学基础部中心实验室,陕西西安710032 [2]兰州军区兰州总医院妇产科,甘肃兰州730050 [3]第四军医大学预防医学系放射医学与防护教研室,陕西西安710032 [4]第四军医大学基础部病理学与病理生理学教研室,陕西西安710032

出　　处：《医学研究生学报》2009年第5期469-471,I0004,共4页Journal of Medical Postgraduates

基　　金：全军医药卫生科技"十一五"面上课题基金资助(批准号:06MA220)

摘　　要：目的：鉴于应激反应与器官损伤的重要关系,文章通过观察三硝基甲苯（TNT）爆炸致伤后羊肝组织超微结构的病理变化特征,为爆炸伤的临床救治提供依据。方法：将25只羊布放在离爆心5~30 m的距离上用电启动引爆TNT致伤,伤后取肝组织用30 mg/L戊二醛固定,进行常规透射电镜标本制备,观察其超微结构病理改变。结果：电镜下肝组织超微结构的病变表现为肝血窦内淤血明显,枯否细胞增生,可见中性粒细胞、淋巴细胞浸润。肝细胞溶酶体增生,髓鞘样膜结构形成,线粒体肿胀、畸形、嵴模糊不清,内质网扩张。在5 m处存活率为0,10、15和20m处分别为40%、60%和80%,25m、30m处动物存活率为100%。结论：爆炸伤后动物存在应激性肝细胞损伤。动物存活率随动物距爆心距离增加而上升。Objective： To observe the uhrastructural pathologlcat characteristics of goat liver tissues affter blast injury from TNT explosion in order to provide an experimental basis for the clinical treatment of blast injury. Methods： Twenty-five goats received blast injury from TNT explosion 5 -30 m from the explosion center. Then their liver tissues were fixed with 3 mg/L glutaraldehyde, and the specimens prepared by routine transmission electron microscopy and observed for ultrastructural pathological changes. Results： We observed obvious congestion in the hepatic sinusoids, proliferation of Kupffer cells and infiltration of neutrophilie granulocytes and lymphocytes. We also found proliferation of lysosomes,formation of myelin-like membrane, swelling and abnormality as well as cisternal mistiness of mitochondria, dilation of endoplasmic reticulum in the hepatocytes. The survival rates were 0% at 5 m, 40% at 10 m, 60% at 15 m, 80% at 20 m and 100% at 25 or 30 m, respectively. Conclusion： Stress-induced damage to hepatocytes occurred in animals with explosive blast injury. The survival rates increased with the increasing of the distance from the explosion center.

关 键 词：三硝基甲苯 爆炸伤 肝细胞 应激 透射电镜 

分 类 号：R642[医药卫生—外科学]