特布他林人工抗原的合成和抗体的制备  

Synthesis of Terbutalin Artificial Antigen and Preparation of Anti-Terbutalin Antibody

作　　者：孙海新 凌红丽 张玉兰[2] 曹照真[2] 高亚东 郗日沫[2] 

机构地区：[1]青岛康地恩实业有限公司,山东青岛266111 [2]山东大学化学与化工学院,山东济南250100

出　　处：《食品与生物技术学报》2009年第3期371-376,共6页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(20675048);国家863计划项目(07AA10Z4352007AA06A407);青岛市科技计划项目(06-3-2-4-nsh)

摘　　要：特布他林是一种在食品中禁用的β-兴奋剂类药物,为建立针对β-兴奋剂的残留快速检测方法,作者研究了特布他林免疫原的合成和抗体的制备方法。采用1,4-丁二醇缩水甘油醚(BDE)、对氨基苯甲酸(ABA)将特布他林(TER)分别与cBSA、cOVA偶联,合成了特布他林免疫原和包被抗原,并对其进行了紫外分析。用特布他林免疫原TER-cBSA免疫新西兰大白兔,获得了多克隆抗体,采用间接ELISA法检测抗体的性质,所得抗体效价达到51 200,IC50值为9.25ng/mL,证明了特布他林人工抗原偶联成功,所制备的特布他林抗体具有很高的灵敏度。In this study, the immunogen of terbutalin was synthesized using 1, 4-butanediol diglycidyl （BDE） as linking reagents to connect hapten terbutalin with carrier protein bovine serum albumin （cBSA）. The coating antigen was synthesized by using 4-aminobenzoic acid （ABA） as linker to connect terbutalin with ovalbumin （cOVA）. The BSA conjugate was used to immunize New Zealand rabbits and polyclonal antibodies against terbutalin were obtained. The titer of antibodies were determined to be 51 200 by indirect ELISA and the antibodies show high sensitivity in the heterologous assay using TER-cOVA as a coating antigen, with an IC50 value of 9.25 ng/mL toward terbutalin. The results indicated that the artificially synthesized antigen has perfect immunogencity and the antibody obtained has excellent quality.

关 键 词：特布他林 ELISA 偶联 抗原 抗体 

分 类 号：TQ463[化学工程—制药化工]