磁场对肌酸激酶的影响  

Magnetic Field Effect on Creatine Kinase

作　　者：钟科军[1] 朱元保[1] 何双娥[1] 曹祉祥[1] 

机构地区：[1]湖南大学化学化工学院

出　　处：《湖南大学学报（自然科学版）》1998年第3期31-35,共5页Journal of Hunan University:Natural Sciences

基　　金：国家自然科学基金

摘　　要：建立了流动注射和光纤光度法测定肌酸激酶（Ｃｋ）活性的方法．求测了Ｃｋ催化反应的米氏常数ｋｍ和反应活化能Ｅ，研究了磁场对Ｃｋ催化活性的影响．较长时间磁化Ｃｋ溶液和测活溶液，分别可使Ｃｋ加速失活和活性升高，１００ｍＴ磁场磁化处理测活液１５ｈ可使Ｃｋ催化反应速度增加４３．７％．Rapid and simple flow injection spectrophotometric and fiberoptic spectrophotometric method have been estabilished for the assay of Ck activity.The michaelis constant and activity energy of enzymic reaction were also determined.The effect of magnetic field on activity of Ck was studied.When the Ck is exposed to magnetic field for a long time,the activity of Ck is decreased accelerately.However,after long magnetizing the substrate solution the activity of Ck is increased.When the substrate solution is exposed to 100 mT magnetic field for 15 h,the rate of the enzymic reaction is increased by 43.7 %.No changes in the rate of the enzymic reaction were observed for periods of exposure of the reaction system in magnetic field.

关 键 词：肌酸激酶 催化活性 磁场 酶催化剂 

分 类 号：Q556[生物学—生物化学] Q689