HPLC测定湖南省地产百合中薯蓣皂苷元含量  

Determine the content of diosgenin in Lily from Hunan province with HPLC

作　　者：黄山林 童巧珍[2] 周日宝[2] 王朝晖[2] 史艳霞[2] 任旻琼[2] 王珊[2] 刘湘丹[2] 

机构地区：[1]耒阳市新型农村合作医疗管理办公室,湖南耒阳421800 [2]湖南中医药大学,湖南长沙410208

出　　处：《湘南学院学报（医学版）》2009年第2期9-11,共3页Journal of Xiangnan University（Medical Sciences）

基　　金：国家科技支撑项目（2006BAI06A11～06）、湖南省中医院管理局资助项目（2008089）

摘　　要：目的测定百合药材中薯蓣皂苷元的含量。方法Phenomenex C18色谱柱（4.6×250mm,5μm）;流速：0.7ml/min;柱温：30℃;检测波长：203nm;流动相：乙腈-水梯度洗脱。结果薯蓣皂苷元线性范围为0.04μg～1.60μg,r=0.9988,回收率为98.16%,RSD为2.07%。结论本方法灵敏、准确,可用于百合药材的质量控制。Objective To detennine the content of diosgenin in Lily. Methods Phenomenex C18 （4.6×250mm,5μm）colour spectntm post was used. Flow rate： 0.7 ml/min; Post temperature： 30 ℃ ; Check wavelength： 203 nm; Grad is washed coming off by mobile phase mixed of acetonitrile - water. Results The linear range of diosgenin was from 0.04 μg- 1.60 μg, r = 0.9988. The recovery of the added sample was 98.16%, RSD was 2.07%. Conclusion The method was simple, sensitive, accurate and be used as one of method for quality control of Lily.

关 键 词：HPLC 百合 薯蓣皂苷元 含量 

分 类 号：R927[医药卫生—药学]