细胞分裂素对大豆叶片衰老过程中蛋白激酶基因表达的影响  

EFFECT OF CYTOKININ ON THE EXPRESSION OF PROTEIN KINASE GENES DURING SOYBEAN LEAF SENESCENCE

作　　者：王宁宁[1] 王勇[1] 王淑芳[1] 朱亮基[1] 张自立[1] 张韧 

机构地区：[1]南开大学生物化学与分子生物学系,天津300071 [2]Wollongong大学生物系

出　　处：《南开大学学报（自然科学版）》1998年第2期99-101,共3页Acta Scientiarum Naturalium Universitatis Nankaiensis

摘　　要：利用真核生物催化丝氨酸／苏氨酸残基磷酸化的蛋白激酶Ⅵ和Ⅷ保守区设计兼并引物，进行RT－PCR扩增反应，研究大豆叶片衰老过程中蛋白激酶基因表达的变化及外源细胞分裂素处理的作用．结果表明人工合成的细胞分裂素6－BA预处理很可能在转录水平上正向调节一些蛋白激酶基因的表达，而诱导衰老处理则起负调控作用．By targeting conserved amino acid sequences from the catalytic domains Ⅵ and Ⅷ of eukaryotic serine/threonine protein kinases for RT-PCR primers design, we studied the expression of protein kinase genes during soybean leaf senescence. The specific products of protein kinase cDNA amplification changed greatly between the samples with or without pretreatment of 6-BA, and the results implied that cytokinin may retard soybean leaf senescence by promoting gene transcription of special protein kinases.

关 键 词：大豆 叶片 蛋白激酶基因 细胞分裂素 植物 衰老 

分 类 号：Q945.48[生物学—植物学]