萤火虫荧光素酶分离纯化及其特性的研究  

Separation and properties of firefly luciferase

作　　者：蒋宇扬[1,2,3] 金振华[1,2,3] 王书锦 王今朝[1,2,3] 马诚 蒋晓琳 

机构地区：[1]清华大学化学系 [2]中国科学院发育生物学研究所 [3]中国科学院应用生态研究所

出　　处：《清华大学学报（自然科学版）》1998年第6期16-19,共4页Journal of Tsinghua University(Science and Technology)

摘　　要：萤火虫荧光素酶只在萤火虫体内产生，一般通过养殖和用化学法提取。研究了以基因工程菌Ｅｓｃｈｅｒｉｃｈｉａｃｏｌｉ－Ｌｕｓ－Ｊ－１为材料，从它的对数生长期发酵液中提取萤火虫荧光素酶。经检测其分子量为５×１０４，动力学性质研究表明它是非典型的Ｍｉｃｈａｅｌｉｓ－Ｍｅｎｔｅｎ氏酶，对荧光素作用动力学曲线为Ｓ型，最适ｐＨ为７．５，最适温度为１５℃，且对光和盐浓度非常敏感。The luciferase can be produced in body and can be obtained by breeding firefly and chemical extract. The luciferase has been purified from fermentative liquid of Escherichia coilLusJ, genetic recombination strain. Its molecular weight is 50000. Dynamic studies demonstrated that it does not behave the character of typical MechaelisMenten enzyme. The dynamics curve of the luciferase for luciferin shows a S shape. Its optimal pH and temperature are 7.5 and 15℃, respectively. It is sensitive to light and salt concentration.

关 键 词：萤火虫 荧光素酶 分离 纯化 特性 

分 类 号：Q966[生物学—昆虫学]