高渗盐水对手术伤口愈合的影响及其免疫机理的探讨  

Effects of treatment with hypertonic saline on cellular immunity and wound healing in mice after surgical operation

作　　者：李汉臣[1] 范雪莉[1] 许利阶 刘先珍[1] 

机构地区：[1]第四军医大学西京医院血管外科

出　　处：《细胞与分子免疫学杂志》1998年第2期139-140,共2页Chinese Journal of Cellular and Molecular Immunology

摘　　要：本研究比较了高渗盐水和生理盐水处理，对手术小鼠细胞免疫功能和伤口愈合的影响。结果表明，高渗盐水处理组小鼠腹壁切口裂开的张力（１４９．０±９．１ｍｍＨｇ）明显高于生理盐水处理组（１２４．１±９．２ｍｍＨｇ）；高渗盐水处理组的迟发型超敏反应（ＤＴＨ）程度（０．０７３ｇ±０．０２５ｇ）和淋巴细胞增殖能力（刺激指数１８．０±９．６）均明显高于生理盐水处理组（分别为０．０４５ｇ±０．００９ｇ和８．５±７．６）。体外实验还发现，提高培养液中氯化钠的浓度，术后２４ｈ淋巴细胞的活性增加。Aim: The experiment was designed to compare the effect of small volume hypertonic saline or isotonic saline treatment on wound healing and cellular immunologic function in mice after surgery. Methods: Animals underwent a standardized midline leparotomy, which was followed by intravenous injection of 7.5% NaCl solution in a volume of 10 ml/kg body weight(HTS group, n =10) or normal saline in a volume of 20 ml/kg body weight (NS group, n =10). The splitting strength (mmHg) of incision and cellular immunological function were measured afterward. Results: The splitting strength of incision in mice of HTS group was 149.1±9.2 mmHg, which was significantly higher than that of mice in NS group (124.1±9.2 mmHg). The delayed type hypersensitivity(DTH) was expressed as the weight of hindlimb foot that was sensitized with DNFB minus the weight of the contralateral control foot. and the lymphocyte proliferation response to PHA was determined using a stimulating index(SI). DTH and SI were significantly elevated in HTS group(0.0725±0.25 g, 18.0±9.6) compared to that in NS group(0.045±0.009, 8.5±7.6). In vitro , hypertonic media elicited obviously higher metabolic activity of lymphocytes obtained from spleen of mice 24 hours after leparotomy in NS group. Conclusion: In contrast to isotonic saline, hypertonic saline could improve wound healing and immunological status in mice afer operation.

关 键 词：高渗盐水 伤口愈合 细胞免疫 外科手术 

分 类 号：R619.5[医药卫生—外科学]