依替膦酸钠片的毛细管电泳-间接UV法测定  

Determination of Etidronate Disodium by Capillary Electrophoresis with Indirect UV Detection

作　　者：安普丽[1] 蒋晔[1] 李海霞[1] 

机构地区：[1]河北医科大学药学院分析化学教研室,河北石家庄050017

出　　处：《中国医药工业杂志》2009年第6期452-454,共3页Chinese Journal of Pharmaceuticals

基　　金：河北省自然基金资助项目(C2007000792)

摘　　要：建立了高效毛细管电泳-间接紫外吸收检测法测定依替膦酸钠。采用未涂层熔融石英毛细管柱,以7mmol/L苯甲酸钠-7mmol/L磷酸二氢钾(pH8.0)为运行缓冲液,分离电压20kV,检测波长224nm。依替膦酸钠在62.5～1000μg/ml浓度范围内线性关系良好,检测限为7.82μg/ml,回收率为98.1%～100.8%,RSD≤1.5%。A capillary electrophoresis method with indirect UV detection was established for the determination of etidronate disodium. An uncoated fused-silica capillary column was used, with the running buffer of 7 mmol/L sodium benzoate-7 mmol/L potassium dihydrogen phosphate （pH 8.0）, at the applied voltage of 20 kV and detection wavelength of 224 nm. The calibration curve was linear in the range of 62.5-1000 μg/ml, with the detection limit of 7.82 μg/ml. The recoveries of etidronate disodium were 98.1%-100.8 %, with the RSDs no more than 1.5 %.

关 键 词：依替膦酸钠 毛细管区带电泳 间接紫外吸收检测 测定 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.8[化学工程—制药化工]