新藤黄酸脂质体冻干粉的制备及其包封率测定  

Preparation of Neo-gambogic lyophilized liposomes and determination of its encapsulation efficiency

作　　者：方玲[1,2] 孟楣[3] 夏伦祝[3] 王效山[1,2] 彭代银[1,2] 

机构地区：[1]安徽中医学院药学院,安徽合肥230061 [2]安徽省中药研究和开发重点实验室,安徽合肥230061 [3]安徽中医学院第一附属医院药学部,安徽合肥230031

出　　处：《安徽医药》2009年第6期596-598,共3页Anhui Medical and Pharmaceutical Journal

基　　金：安徽省十一五科技攻关项目(No06013131B)

摘　　要：目的制备新藤黄酸脂质体冻干粉并测定其包封率。方法采用薄膜-超声法结合冷冻干燥法制备新藤黄酸脂质体冻干粉,以冷冻超速离心-高效液相色谱法测定包封率。结果电镜下观察所制备的脂质体形态规整,平均粒径为128.30 nm,包封率为83.74%。结论此法制备了包封率较高的新藤黄酸冻干脂质体。Aim To prepare Neo-gambogic lyophilized Liposomes and to develop a method for determining its entrapment efficiency. Methods Liposomes were prepared by rotary-evaporated film-ultrasonication method; and then further treated with lyophilization. Free GNA and liposomes were separated by uhracentrifugation and HPLC was applied to determine the encapsulation efficiency. Results GNA liposomes were uniform and spheric; its mean particle size was 128.30 nm; the encapsulation efficiency was 83.74%. Conclusion The present study had prepared GNA liposomes with the property of high encapsulation efficiency.

关 键 词：新藤黄酸 脂质体 冷冻干燥 包封率 

分 类 号：TQ461[化学工程—制药化工]