巨龙竹AINTEGUMENTA基因的克隆研究  

Cloning of AINTEGUMENTA Gene with RACE from Dendrocalamus sinicus

作　　者：张绍智[1] 刘小烛[1] 

机构地区：[1]西南林业大学资源学院,云南昆明650224

出　　处：《竹子研究汇刊》2009年第1期16-20,共5页Journal of Bamboo Research

基　　金：国家林业局西南地区生物多样性保育重点实验室开放基金资助(KL200704);云南省省级重点建设专业西南林学院生物技术专业资助

摘　　要：为进一步探索巨龙竹速生与巨大的分子机理,研究其AINTEGUMENTA(ANT)基因功能,研究试验以巨龙竹cDNA第一链为模板,用简并引物、基因特异引物以及运用RACE技术首次克隆出巨龙竹ANT基因全长cDNA序列。其序列全长为1 914 bp,与水稻、玉米、小麦等植物同类基因序列相似性高达85%;并且含有一个837 bp的开放阅读框,编码278个与水稻、拟南芥和烟草ANT蛋白氨基酸序列有着较高同源性的巨龙竹ANT前体蛋白氨基酸序列。In order to study the molecular mechanism of rapid growth and hugeness in Dendrocalamus sinicus, and investigate the function of AINTEGUMENTA （ANT） gene, we took the first cDNA chain of Dendrocalamus sinicus as template, use the degenerating primer and the gene-specific primer, and finally cloned the total cDNA sequence of ANT gene by RACE method. The total cDNA has 1 914 bp, sharing high similarity with the cDNA ANT of Oryza sativa, Triticum aestivurn and Zea roans; Moreover, it includes an Open Reading Frame witch code 278 prior amino acid of ANT, and the amino acid has high consanguinity with that of ANT of Oryza sativa, Arabidopsis thaliana and Nicotiana tabacum.

关 键 词：巨龙竹 AINTEGUMENTA RACE CDNA 克隆 

分 类 号：S795[农业科学—林木遗传育种] Q78[农业科学—林学]