PCR熔解曲线法检测HBV YMDD自然变异  

Detection of spontaneous hepatitis B virus YMDD motif mutation by fluorescent hybridlzation blprobe PCR and melting curve assay

作　　者：唐孝亮[1] 

机构地区：[1]湖南省怀化市第一人民医院,湖南怀化418000

出　　处：《中国现代医学杂志》2009年第10期1544-1546,共3页China Journal of Modern Medicine

摘　　要：目的了解未经抗病毒治疗的慢性乙型肝炎(CHB)患者乙肝病毒YMDD自然变异流行状况及特点。方法选择未接受过抗病毒治疗的慢性乙型肝炎患者119例,采用荧光标记杂交双探针PCR熔解曲线法(FH-PCR-MC)对其血浆标本进行YMDD基因序列突变检测。结果119例患者中,有23例存在YMDD自然变异毒株,变异检出率为19.3%,其中YVDD阳性者20例(87.0%),YIDD阳性者2例(8.7%),YVDD和YIDD同时阳性者1例(4.3%)。23例出现自然变异毒株的患者,其体内病毒均为变异株与野生株共生,且变异株为非优势株,变异株在总病毒量中所占的比例均在50%以下。变异毒株占总病毒量的40%～50%者4例,30%～40%者6例,20%～30%者6例,20%以下者7例。结论未经抗病毒治疗的CHB患者存在YMDD自然变异毒株,且变异株与野生株共生,为非优势株。[Objective] To investigate the natural mutation of hepatitis B virus （HBV） YMDD motif in the patients with chronic hepatitis B （CHB） who were not treated with lamivudine, and to observe its epidemiological characteristic. [Methods] The natural mutation of HBV YMDD motif was detected by fluorescent hybridization blprobe PCR and melting curve assay （FH-PCR-MC） in 119 patients with CHB. [Results] Among 119 subjects with CHB, spontaneous YMDD variants were detected in 23 subjects （YVDD, YIDD and YVDDfYIDD for 20, 2 and 1 subject, respectively）, and were all found to coexist with wild-type virus. YMDD variants account for 40%-50%, 30%-40%, 20%-30%, 〈20% of total virus load in 4, 6, 6, 7 subjects,respectively; [Conclusions] There are natural mutants of HBV YMDD in the patients with CHB who were not treated with lamivudine. And natural mutants of HBV YMDD were all found to coexist with wild-type virus.

关 键 词：乙型肝炎病毒 YMDD 自然变异 聚合酶链反应 熔解曲线法 

分 类 号：R512.62[医药卫生—内科学]