华南汉族心肌梗死患者凝血因子VII R353Q基因型的检测  

Association of coagulation factor VII gene polymorphisms with myocardial infarction patients of Han nationality in south of China

作　　者：黄志军 王水云 甘美连 李体远 朱莹 林秀华 黄璐璐 柏承文 

机构地区：[1]广东省深圳市人民医院(暨南大学第二临床医学院),广东深圳518020

出　　处：《中国现代医学杂志》2009年第10期1575-1577,共3页China Journal of Modern Medicine

基　　金：深圳市科技局项目(No:200405020);暨南大学医学院项目(No:WSTJJ20030108440301510812251)

摘　　要：目的观察凝血因子II(CFVII)R353Q基因多态性在华南汉族人群中的分布及其与心肌梗死MI患者的关系。方法提取华南汉族78例心肌梗死患者和60名正常对照者人基因组DNA,应用多聚酶链反应(PCR)技术和限制性内切酶片段长度多态性技术检测上述人群的CFVII R353Q基因型,分析正常人群及心肌梗死者基因型频率的不同。结果华南汉族人群凝血因子CFVII基因R353Q多态具3种基因型(RR,RQ,QQ),CFVII等位基因R、Q基因频率在心肌梗死组和对照组分别为97.4%、2.6%和90.9%、9.1%。基因型频率符合Hardy-Weinberg平衡定律。R353Q等位基因频率在心肌梗死组和对照组之间比较差异有显著性。MI患者R等位基因携带频率明显高于对照组。结论CFVII R353Q中Q等位基因对华南汉族心肌梗死的发生有一定的保护作用。凝血因子CFVII基因R353Q多态的遗传性差异在华南汉族MI发病中可能具有重要作用。[Objective] To study the distribution of coagulation factor VII gene polymorphisms in nationality Han and the association of the polymorphisms with myocardial infarction in South China. [Methods] Genotypes of FVII were typed in 78 myocardial infarction patients and 60 controls by polymerase chain reaction-restriction fragment length polymorphism. [Results] CFVII allelic frequencies of R, Q were 97.4%, 2.6% and 90.9%, 9.1% in myocardial infarction group and control group respectively. Genotype distribution was in accordance with Hardy-Weinberg equilibrium. There was statistically significant difference in frequencies of allele in R353Q polymorphisms between myocardial infarction group and control group. [Conclusion] The R353Q polymorphisms of the FVII gene have a relationship with myocardial infarction patients of Hart nationality in south China.

关 键 词：心肌梗死 凝血因子VII 基因多态性 华南汉族 

分 类 号：R446[医药卫生—诊断学]