乙型肝炎病毒感染与小儿膜性肾炎  

HBV infection and childhood membranous glomerulonephritis

作　　者：郭怡清[1,2] 方利君[1,2] 张月娥 盛芳芸[1,2] 郭慕依[1,2] 贺新玉[1,2] 

机构地区：[1]上海医科大学儿科医院 [2]上海医科大学病理教研室

出　　处：《中华肾脏病杂志》1998年第3期159-162,共4页Chinese Journal of Nephrology

摘　　要：目的研究ＨＢＶ感染在小儿膜性肾炎的致病作用。方法３２例肾穿刺活组织检查诊为膜性肾炎的小儿作为研究对象。所有病例检测血清ＨＢＶ感染标志物，２９例应用免疫组化技术检测肾组织ＨＢＶ抗原，１２例应用Ｓｏｕｔｈｅｒｎ印迹杂交检测肾组织ＨＢＶＤＮＡ，采用３２Ｐα－ｄＣＴＰ标记的探针；２８例应用原位杂交检测肾组织ＨＢＶＤＮＡ，采用地高辛标记的探针。结果三种方法综合分析，３２例膜性肾炎中，肾组织ＨＢＶ抗原和（或）ＨＢＶＤＮＡ阳性者共３０例，达９３．７４％。Objective To investigate the role of HBV in the pathogensis of childhood membranous glomerulonephritis (MGN).Methods 32 cases of MGN were studiedSerum markers of HBV infection were determined in all cases,29 of them with HBV antigenemia(9062%)HBV antigens were detected by immunohistochemical techniques in renal tissue in 24 out of 29 casesHBV DNA were examined in 12 cases by Southern blot hybridization with32 PαdCTP labelled HBV DNA probe,HBV DNA was present in 11 cases (9166%), 9 of them were identified as integrated form while 2 cases were of nonreplicating free formIn addition,HBV DNA were observed in 28 cases by in situ hybridization using digoxigenin labelled HBV DNA prode,in 26 of them HBV DNA was detectable (9285%)in the nucleus of glomerular epithelial cells,mesangial cells and matrix,in 13 cases it was simultaneously found in the nucleus of tubular epithelial cellsResults There were 30 cases of MGN showing positive results for HBV antigen and/or HBV DNA in renal tissue(9374%).Conclusion MGN in children is closely related to HBV infection in China

关 键 词：肾炎 膜性肾炎 乙型肝炎病毒 感染 儿童 

分 类 号：R726.923[医药卫生—儿科] R725.126.2[医药卫生—临床医学]