微滴中胚胎数量对猪孤雌胚早期体外发育的影响  

Effect of Embryo Number in Micro-drops on Early Vitro Development of Porcine Parthenogenetic Embryos

作　　者：罗龙兴[1,2,3] 何若钢[2] 卢晟盛[1,2] 潘天彪[3,4] 蓝海恩[3,4] 黄敏瑞[3,4] 卢克焕[1,2] 

机构地区：[1]广西亚热带生物资源保护利用重点实验室,广西南宁530004 [2]广西大学动物科技学院,广西南宁530004 [3]广西柳州畜牧兽医学校,广西柳州545003 [4]广西畜牧研究所,广西南宁530001

出　　处：《安徽农业科学》2009年第16期7448-7449,共2页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(30660127);广西科学研究与技术开发计划项目(桂科能0815011-6-3);广西亚热带生物资源保护利用重点实验室开放课题基金项目(SB0701)

摘　　要：[目的]优化猪早期胚胎培养体系,为单精子显微受精及体细胞核移植等研究提供依据。[方法]以猪的早期孤雌胚胎为材料,分别将人工激活后的70、50、30、15和5枚卵母细胞放入100μlNCSU-23培养液的微滴中进行培养,探讨了胚胎培养数量对猪早期孤雌胚发育的影响。[结果]100μl的培养微滴中培养30、50和70胚胎组,其分裂率分别为64.0%、65.2%和67.1%,显著高于5胚胎组,而与15胚胎组的差异不显著 在囊胚率方面,70胚胎组的最高,为3.0%,与50胚胎组的（1.7%）无显著差异,与5、15和30胚胎组的差异显著 各组的囊胚细胞数之间没有显著差异。[结论]在该研究条件下,当微滴体积为100μl时,培养50~70枚猪孤雌胚胎的效果最好,即,胚胎数∶培养滴体积=（1∶1.43~2.00）。[ Objective ] The aim was to optimize the early embryo culture system of porcine and provide the basis for the research of single sperm micro fertilization and somatic cell nuclear transplantation etc. [ Method] With the early parthenogenetic embryos of porcine as the tested material, the 70, 50, 30, 15 and 5 pieces of oocytes with artificial activation were put into the micro drop of 100μl NCSU-23 culture liquid resp. to culture and the effect of embryo culture number on early vitro development of porcine parthenogenetic embryos was discussed. [ Resuit] When the 30, 50 and 70 embryo groups were cultured in 100μl culture micro drop, their cleavage rate were 64.0% , 65.2% and 67.1% resp. , and were obvious higher than that in 5 embryo groups, and their difference were not remarkable with that in the 15 embryo groups. The 70 embryo groups was highest on the blastoeyst rate, reaching 3.0% , which was not remarkably different when comparing with that in 50 embryo groups （ 1.7% ） and was remarkably different comparing with that in 5, 15 and 30 embryo groups, but the blastocyst cell number of each group had not remarkable different. [ Conclusion] Under this study, when the medium micro-drop volume was 100 μl, culturing 50- 70 pieces of parthenogenetic embryo could get best effects that is the embryo number： the bulk of culture drop = 1：（ 1.43 -2.00）.

关 键 词：猪 卵母细胞 孤雌激活 胚胎培养 

分 类 号：S812[农业科学—草业科学]