内皮抑素在大鼠腹膜的表达及其与腹膜血管新生的关系  

Expression of endostatin in rat peritoneum and its association with peritoneal neoangiogensis

作　　者：赵占正[1] 刘章锁[1] 李艳[1] 高丹[1] 梁献慧[1] 

机构地区：[1]郑州大学附属第一医院肾内科,450052

出　　处：《中华肾脏病杂志》2009年第6期437-440,共4页Chinese Journal of Nephrology

摘　　要：目的研究大鼠腹膜血管内皮抑素（endostatin，ES）基因及蛋白表达与腹膜血管新生的关系。方法32只雄性SD大鼠，按随机数字表法分为正常组、肾衰竭非透析组（非腹透组）、1．5％腹膜透析组（1．5％PD组）、4．25％腹膜透析组（4．25％PD组），每组8只。PD组经规律PD28d后，取各组大鼠新鲜腹膜组织，用RT—PCR检测ESmRNA表达；用组织免疫组化染色检测ES蛋白表达；以CD34染色观察腹膜组织毛细血管密度（MVD）。结果各组均有ESmRNA表达，正常组为0．47±0．05；非腹透组为0．45±0．04；1．5％PD组为0．46±0．04；4．25％PD组为0．47±0．03；各组表达差异无统计学意义。正常组ES蛋白表达积分0分；非腹透组积分2分；1．5％PD组积分4分；4．25％PD组呈高表达，积分9分。正常组MVD为3．13±1．13；非腹透组为5．13±1．14；1．5％PD组为9．00±1．51；4．25％PD组为10．75±1．83；组间差异均有统计学意义（P〈0．05）。结论尿毒症状态和非生理性腹透液刺激可使大鼠腹膜组织ES蛋白表达升高，其在长期透析所致腹膜组织毛细血管生成增多中可能发挥一定的作用。Objective To investigate the expression of endostatin （ES） in rat peritoneum and its association with peritoneal neoangiogensis. Methods Thirty-two male SD rats were randomly divided into 4 groups： normal control rats （C group）, renal failure without PD rats （non- PD group）, rats dialyzed with 1.5% PD solution （1.5% PD group） and 4.25% PD solution （4.25% PD group）. After regular PD for 28 days, mRNA and protein expression of ES in peritoneal tissues of each group were detected by RT-PCR and immunobistoehemistry respectively. Mierovessel density （MVD） of peritoneal tissue was assessed using immunohistochemistry with CD34 monoclonal antibody. Results ES mRNA was expressed in each group, 0.47 ±0.05 in C group, 0.45± 0.04 in non-PD group, 0.46±0.04 in 1.5%PD group, 0.47±0.03 in 4.25%PD group, and no significant differences were found among groups. Score of ES protein expression was 0 in C group, 2 in non-PD group, 4 in 1.5%PD group, and 9 in 4.25%PD group. MVD was 3.13±1.13 in C group, 5.13±1.14 in non-PD group, 9.00±1.51 in 1.5%PD group, 10.75±1.83 in 4.25%PD group, and significant differences were found among groups. Conclusion Uremia circumstance and non-physiological compatibility peritoneal dialysate can increase ES protein expression and MVD, which may participate in and have effects on the course of peritoneal neoangiogensis.

关 键 词：内皮抑素类 腹膜透析 新生血管化 病理性 毛细血管密度 

分 类 号：R692.5[医药卫生—泌尿科学]