中国口蹄疫病毒同源重组研究  

A Study of Homologous Recombination in Foot-and-mouth Disease Virus in China

作　　者：武治印[1,2] 何成强[3] 刘莹莹[1] 冯倩[1] 滕俊琳[1] 陈建国[1,2] 

机构地区：[1]北京大学生命科学学院细胞生物学和遗传学系,细胞增殖与分化教育部重点实验室,生物膜及膜生物工程国家重点实验室,北京100871 [2]北京大学理论生物学中心,北京100871 [3]山东师范大学生命科学学院,山东省动物抗性生物学重点实验室,济南250014

出　　处：《生物化学与生物物理进展》2009年第6期689-695,共7页Progress In Biochemistry and Biophysics

摘　　要：从GenBank数据库中获得在我国分离的16株口蹄疫病毒全基因组序列,进而运用常规的系统发生方法分析了这16株病毒的同源重组情况,发现5株重组毒株.这些重组病毒主要来源于亚洲Ⅰ型(Asia1)和O型病毒间的重组.这些重组事件的鉴定也表明口蹄疫病毒间的交叉感染在我国比较常见.另外,在我国还出现了由于Asia1型和O型病毒重组后导致病毒血清型发生转化的现象.这些结果解释了我国口蹄疫病毒(FMDV)遗传多样性和抗原多变性的成因,提示了我国在口蹄疫预防、治疗方面所面临的复杂局面.Foot-and-mouth disease virus （FMDV） is a positive-sense RNA virus which has caused severe damage to world-wide livestock industry. The extensive genetic and antigenic diversity observed in the evolution of FMDV is generally the obstacle for controlling the disease. The homologous recombination, as a significant force driving the evolution of virus, has also effect on the epidemiological trait of FMDV. However, the role of homologous recombination in the diversification of FMDV in China has not investigated. So it is necessary to study the homologous recombination underlying the evolution of FMDV to control FMD. Based on a sound evolutionary framework, molecular evolutionary analysis was used to identify the putative recombinants. All complete FMDV genomes from China were respectively retrieved from GenBank. Homologous recombination was identified using Simplot program. Phylogenetic relations were analyzed to determine the recombination events among these FMDV isolates by using MEGA 4. The isolates O/NY00, O/China/1/99Tibet, O/Tibet/CHA/99, O/OMIII and O/ES/2001 among 16 FMDVs were identified as putative recombinants by analyzing the FMDV genomic sequences extracted from GenBank. The recombination events frequently happen between serological type Asial and O which are endemic FMDV circulating in China, suggesting frequent cross infection of FMDV in China. This situation further makes controlling FMDV in China more difficult. Moreover, serotypic conversion of FMDV between Asial and O was detected to be due to homologous recombination. These results provided clues for understanding the antigenic and genetic diversification in FMDV, and shed lights on the potential vaccination and treatment of FMD.

关 键 词：口蹄疫病毒 同源重组 进化 

分 类 号：Q811[生物学—生物工程]