以软刻技术微图案化培养纹状体神经元的形态学研究  

A Morphologic Study on Creation of Neural Network of Cultured Striatal Neurons in vitro Using Soft Lithography Techniques

作　　者：高侃[1] 刘丙方[1] 徐群渊[1] 

机构地区：[1]首都医科大学北京神经科学研究所,北京市神经再生修复研究重点实验室,教育部神经变性病学重点实验室,北京100069

出　　处：《生物化学与生物物理进展》2009年第6期787-792,共6页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目(30700210)~~

摘　　要：探讨以软刻技术微加工带正电荷的聚乙烯亚胺(polyethyleneimine,PEI)图案,对培养纹状体神经元黏附存活及突起生长的影响.以软刻技术(微接触印刷方法)微加工三种不同的黏附底物:层粘连蛋白(laminin,LN)、带正电荷的多聚赖氨酸(poly-L-lysine,PLL)和PEI.新生乳鼠纹状体神经元体外分离培养,观察不同黏附底物对纹状体神经元黏附、存活和突起生长状态的影响,观察神经元在不同黏附底物上形成网络图案的差异.结果表明,在PEI和PLL表面生长的神经元数量明显大于LN组,PEI与PLL、LN相比能形成更为完整的神经元图案.带强正电荷的PEI有助于神经元在其表面形成相对完整的图案,是构建体外神经网络的一种良好界面材料.To investigate the attachment and neurite growth of pattemed striatal neurons on the surface of polyethyleneimine （PEI） in vitro, by a soft lithography technique. Three different substrates, including Laminin （LN）, poly·L-lysine（PLL） and PEI characterized with strong positive surface charges were micro-patterned using soft lithography techniques. Striatal neuroris from postnatal rats were cultured in vitro. The condition of cell adhesion, living and the neurite growth of the cultrued neurons on different substrates were observed, and the differences of neural network patterns fabricated on the three substrates were evaluated. The quantity of neuronal growth on the surface of PEI and PLL is apparently larger than that on LN. Moreover, the coated pattern of neurons on the PEI is more integrated than that on the PLL and LN. The PEI characterized with strong positive surface charges is capable of well fabricating more continuous and integrated neural patterns, which is an ideal interracial surface to realize the artificial neural network in vitro.

关 键 词：聚乙烯亚胺 软刻技术 纹状体神经元 细胞培养 

分 类 号：R392.12[医药卫生—免疫学] R319[医药卫生—基础医学]