油茶白朽病菌ITS基因的克隆及序列分析  

Cloning and Sequence Analysis on ITS Gene of Pathogens of White Rot Disease of Camellia oleifera

作　　者：李河[1] 郝艳[1] 宋光桃[1] 赵胜利[2] 何末军[1] 周国英[1] 

机构地区：[1]中南林业科技大学资源与环境学院,湖南长沙410004 [2]怀化市鹤城区林业局,湖南怀化418000

出　　处：《西南林学院学报》2009年第2期40-43,共4页Journal of Southwest Forestry College

基　　金：国家“十一五”林业科技支撑计划项目(2006BAD08A1104)资助;国家林业局重点项目(2006-47,2007-07)资助;湖南省教育厅科研项目(08C930)资助;中南林业科技大学青年科学研究基金项目(07003B)资助;中南林业科技大学研究生科技创新基金项目(2007bx02)资助

摘　　要：分离油茶白朽病的病原菌,并对该菌的ITS基因进行克隆和测序;将所得序列在Genbank中经B lastn搜索,构建分子系统发育树。结果表明:病原菌ITS序列与Genbank数据库中已有的序列同源性较低,最高仅为94%,系统发育树也显示该菌不与其他菌株聚类而是单独聚为1支。由于Genbank数据库有限,难以根据同源性来确定其属种关系。White Rot Disease has caused serious damage to Camellia oleifera. The pathogens of the disease were isolated and the ITS sequences of the pathogens were sequenced and analyzed. The molecular phylogenetic tree was constructed based on Blastn reference to the sequence in the Genbank. The results revealed that rDNA ITS sequence of the pathogen was comparatively low in homology, the highest homology was only 94%. The pylogenetic tree also showed that the pathogen strain was far away from any other strains. It was hard to determine the taxonomic relationship of the pathogens just by the homology because of the limited data in the Genbank.

关 键 词：油茶 白朽病 病原菌 转录间隔区基因 克隆 测序 

分 类 号：Q785[生物学—分子生物学]