杜仲对UUO模型大鼠肾纤维化TGF-β_1/Smad信号通路的影响  

Effects of Eucommia on TGF-β_1/Smad Signaling Pathway in Renal Interstitial Fibrosis in Rats with Unilateral Ureteral Obstruction

作　　者：程虹[1] 宋恩峰[1] 

机构地区：[1]武汉大学人民医院中西医结合科,武汉430060

出　　处：《中国中西医结合肾病杂志》2009年第6期502-504,I0002,共4页Chinese Journal of Integrated Traditional and Western Nephrology

摘　　要：目的:研究杜仲对单侧输尿管梗阻(UUO)大鼠肾组织转化生长因子-β1(TGF-β1)、Smad2、Smad7表达的影响。方法:将32只大鼠随机分为4组,每组8只,即假手术组、模型组、厄贝沙坦组和杜仲组。厄贝沙坦组给予厄贝沙坦10mg·kg-1·d-1、杜仲组给予杜仲6g·kg-1·d-1治疗,均术前2d开始给药。术后2周处死大鼠,收集血清测定尿素氮(BUN)、血肌酐(Scr),光镜下观察Masson和HE染色肾间质的病理改变,并应用免疫组织化学方法检测肾组织TGF-β1和Smad2、Smad7蛋白的表达。结果:模型组与两治疗组大鼠血清Scr、BUN上升,与假手术组比较差异有统计学意义(P<0.05);模型组、杜仲组、厄贝沙坦组肾间质纤维化程度均较假手术组明显增高(P<0.05);模型组TGF-β1、Smad2的表达增加,与假手术组相比差异有统计学意义(P<0.01);杜仲组与模型组相比,TGF-β1、Smad2的表达下降,而Smad7的表达增高,差异有统计学意义(P<0.05);杜仲组与厄贝沙坦组各指标比较差异均无统计学意义(P>0.05)。结论:杜仲能通过下调TGF-β1、Smad2的表达及增加Smad7的表达而抑制单侧输尿管梗阻大鼠肾间质纤维化,机制与杜仲可以部分调节TGF-β1/Smad信号转导通路有关,其改善肾纤维化作用与厄贝沙坦无明显差异。Objective： To study the effects of Eucommia on TGF-β1, Smad2 and Smad7 expressions of renal interstitial fibrosis in Rats with unilateral ureteral obstruction. Methods： Adult male SD rats（ n = 32 ） were randomly divided into four groups： Sham operation group （Sham）, UUO group, Irbesartan group（ 10 mg· kg^-1· d^-1 ） and Eucommia group（6 g· kg^-1· d^-1 ）. The rats in normal group were treated with normal saline by intragastric administration, and the other groups were to establish renal interstitial fibrosis model by unilateral ureteral obstruction （UUO）. The renal tissues were stained by Masson and HE to observe the renal interstitial fibrosis index. The expression of TGF-β1, smad2 and smad7 were detected by immunohistochemistry 2 weeks after surgery. Results： In comparison with that in Sham operation group, the level of TGF-β1 was significantly decreased after treatment with Eucommia. Meanwhile, the level of Smad2 was also down - regulated. However, the level of Smad7 expression was significantly up - regulated. The pathological changes of renal tissues in Irbesartan groups were also markedly alleviated. Conclusion： Eucommia has significant inhibitory effects on renal interstitial fibrosis in rats with unilateral ureteral obstruction, and its mechanism may be associated with the inhibition of TGF -β1 expression, down - regulation of Smad2 expression and up - regulation of Smad7 expression.

关 键 词：杜仲 TGF-β1/Smad通路 肾间质纤维化 

分 类 号：R285.5[医药卫生—中药学]