血管束植入组织工程骨修复兔股骨缺损对血管内皮生长因子表达的影响  

Up-regulated release of vascular endothelial growth factor in tissue engineered bone with implanted vascular bundles： a model of femoral defect in rabbits

作　　者：王簕[1] 覃俊君[1] 陈思园[1] 穆天旺[1] 江汕[1] 赵培冉[1] 金丹[1] 裴国献[1] 

机构地区：[1]南方医科大学南方医院创伤骨科,广州510515

出　　处：《中华创伤骨科杂志》2009年第6期540-545,共6页Chinese Journal of Orthopaedic Trauma

基　　金：基金项目：国家自然科学基金广东省联合重点项目（u0732003）;国家自然科学基金（30600643,30872638）

摘　　要：目的探讨血管束植入组织工程骨修复兔股骨缺损对局部血管内皮生长因子（VEGF）表达的影响。方法将兔自体骨髓基质干细胞经诱导后与β-磷酸三钙材料复合，植入制备的兔股骨缺损处，其中实验组在材料侧槽中植入股骨的动静脉血管束，对照组则单纯植入组织工程骨，分别于术后2、4、8、12周通过形态学检测新生骨量，免疫组织化学方法检测新生骨中VEGF的表达量。结果随着时间进展，各组成骨量逐渐增加，差异有统计学意义（P〈0．05），并且从第4周开始实验组成骨量高于对照组，差异有统计学意义（P〈0．05）；各时间点实验组新生骨中VEGF的表达量均高于对照组，差异有统计学意义（P〈0．05），且4周时达到最大值。结论血管束植入组织工程骨可明显增加新生骨中VEGF的表达并能促进骨缺损的修复。Objective To investigate whether tissue engineered bone with implanted vascular bundies can up-regulate release of vascular endothelial growth factor （VEGF） in models of femoral defect in rabbits. Methods Thirty-two rabbits were randomized into 2 even groups. In both groups, a segmental bone defect of 15 mm in length was made at the left femur before a tissue engineered bone was inserted into the defect. In the experimental group, a femoral vascular bundle was implanted into the tissue engineered bone. In the control group, there was no vascular implantation. At 2, 4, 8, and 12 weeks after implantation, samples were taken to determine new bone formation by histology and expression level of VEGF by immunohistochemistry. Results The new bone formation was significantly higher in the experimental group at the end of 4, 8, and 12 weeks（ P 〈 0. 05） . The expression level of VEGF in the experimental group was also significantly higher than in the control group at all time points after operation, and the expression of VEGF peaked at 4 weeks. Conclusion Tissue engineered bone with vascular bundle implanted can up-regulate VEGF release in models of femoral defect in rabbits.

关 键 词：组织工程 骨 血管化 骨缺损 血管内皮生长因子 

分 类 号：R686[医药卫生—骨科学]