鸭瘟灭活疫苗种毒筛选及生产用种毒种子批的建立  

Establishment of master stocks for preparation of inactivated vaccine against duck plague

作　　者：范书才[1] 李虹[1] 朱良全[1] 康凯[1] 

机构地区：[1]中国兽医药品监察所,北京海淀100081

出　　处：《中国预防兽医学报》2009年第6期458-461,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家科技基础条件平台建设项目(2005DKA21205)

摘　　要：本研究通过鸭胚(DE)传代,建立了鸭瘟病毒(DPV)AV1221株的鸭胚适应毒。通过与其他2株DPV(AV1222株鸡胚毒和AV18株鸭胚毒)免疫原性、毒力等特性的比较,选择AV1221株DE2代鸭胚毒作为制苗用种毒,NJ株D5代鸭肝组织毒作为检验用强毒。使用鸭胚对AV1221株DE2毒进行了连续6次传代。对DE2代～DE7代冻干种毒的鉴定结果表明:未发现细菌、霉菌、支原体和外源病毒污染;病毒含量为每0.2mL含104.38～105.25ELD50;能够被DPV抗血清中和;制成灭活疫苗,免疫成鸭后均能够产生完全保护。依据试验结果建立了能够满足疫苗生产所需的生产用种毒的种子批。Comparing with the other two duck plaque virus （DPV） strains for immunogenicity and virulence, the duck embryosadapted DPV AV1221 （AV1221 DE2） virus strain selected as the master stock. AVI221 DE2 virus was passed in duck embryos for 6 passages in succession and each passage was lyophiled. The virus titers ranged from 10^4.38 to 10^5.25 ELD50 per 0.2 mL and the viruses could be neutralized by DPV antiserum. The vaccines prepared with the viruses induce complete protection in ducks after challenge.

关 键 词：鸭瘟 鸭瘟病毒 灭活疫苗 

分 类 号：S852.52[农业科学—基础兽医学]