牵张力作用下甲状旁腺激素相关蛋白对人成骨样细胞增殖的影响  

Effect of parathyroid hormone related protein on proliferation of human osteoblast-like cell under tension force

作　　者：黄生高[1] 钟孝欢[1] 王会欣[1] 王明朗[1] 

机构地区：[1]中南大学湘雅二医院口腔科,湖南长沙410011

出　　处：《华西口腔医学杂志》2009年第3期344-347,352,共5页West China Journal of Stomatology

摘　　要：目的研究牵张力作用下甲状旁腺激素相关蛋白(PTHrP)对人成骨样细胞增殖的影响。方法建立体外培养细胞牵张力施力模型。利用逆转录聚合酶链反应(RT-PCR)法检测PTHrP mRNA的表达及12%牵张应变作用下PTHrP(1nmol/L)对MG-63细胞c-fos mRNA表达的影响。检测不同浓度PTHrP(0、0.01、0.1、1nmol/L)及12%牵张应变联合作用12h后对细胞增殖活性的影响。结果不同牵张力对PTHrP mRNA表达影响的差异具有统计学意义,12%牵张应变组作用的影响最为显著。牵张力联合PTHrP刺激下培养较PTHrP或牵张力的单独效应具有更强的促MG-63增殖作用。牵张力联合PTHrP作用较牵张力的单独效应具有更强的增加MG-63细胞c-fos mRNA表达的作用。结论牵张力可显著影响人成骨样细胞PTHrP mRNA的表达水平。PTHrP在牵张力环境下较牵张力的单独效应具有更强的促MG-63增殖作用。在牵张力环境下PTHrP可能通过c-fos信号传导途径影响成骨细胞增殖。Objective To investigate the effect of parathyroid hormone related protein （PTHrP） on proliferation of human osteoblasts （MG-63） under the circumstance of tension force in vitro. Methods An apparatus was designed and fabricated by which force was loaded onto the cultured cells in vitro. Reverse transeription-polymerase chain reaction （RT-PCR） was used for measuring the expression of PTHrP mRNA and c-los mRNA. The effect of tension force and different PTHrP dose（0, 0.01, 0.1, 1 nmol/L） on the proliferation of human osteoblasts were examined using flow eytometry. Results Various forces of the mechanical stretching exerted different influences on the intensities of the mRNA＇ expression. The strain of 12% induced the most remarkable mRNA＇ expression. The mitogenesis happened in the group with tension force（12%） combined with PTHrP was more active than that in the group with PTHrP or tension force only. Tension force combined with PTHrP induced significantly more c-fos mRNA than that of tension force only. Conclusion The mechanical stretching can inevitably influence the expression of PTHrP mRNA. The most active mitogenesis happened in the group with tension force combined with PTHrP. The effect may be related with the signaling pathways of c-fos.

关 键 词：张应力 甲状旁腺激素相关蛋白 增殖 

分 类 号：R318.01[医药卫生—生物医学工程]