荧光原位杂交技术分析不育男性精子X、Y及18号染色体  

An Analysis on Chromosome X,Y and 18 of Sperms by Fluorescence in-Situ Hybridization in Infertile Men

作　　者：张云山[1] 杨清秀[1] 罗海宁[1] 糜若然[1] 

机构地区：[1]天津医科大学总医院妇产科,300052

出　　处：《天津医药》2009年第6期439-441,共3页Tianjin Medical Journal

基　　金：天津市科技发展计划项目(项目编号:05YFGZGX09900)

摘　　要：目的:应用荧光原位杂交技术分析不育男性精子的X、Y和18号染色体非整倍体情况。方法:15例接受卵细胞浆内单精子注射(ICSI)治疗的男性不育症患者中严重少弱精子症组(A组)、轻度少弱精子症组(B组)和弱精子症组(C组)各5例。选用CEPX/Y、CEP18染色体探针对其ICSI后剩余的精子进行荧光原位杂交实验,比较3组患者精子染色体的非整倍体发生率。结果:共计数精子3000个,杂交率为97%。A、B、C组染色体非整倍体率分别为(6.72±2.64)%、(1.60±1.02)%和(4.03±1.50)%,差别有统计学意义(F=9.62,P<0.01)。A组、B组之间和A组、C组之间差别均有统计学意义(均P<0.01),B组和C组差别无统计学意义(P>0.05)。结论:对少弱精子症患者尤其是严重少弱精子症患者进行ICSI治疗时,应该在胚胎移植前进行胚胎染色体非整倍体筛查,防止染色体非整倍体胎儿出生。Objective： To analyze the rate of aneuploid sperms on chromosome X,Y and 18 by fluorescence in-situ hybridization in infertile men. Methods： Fifteen infertile men who received intracytoplasmic sperm injection（ICSI） were dividend into three groups, including 5 patients with severe oligo-asthenospermia （group A）, 5 patients with mild oligo-asthenospermia （group B） and 5 patients with asthenospermia （group C）. The sperms were processed by fluorescence in-situ hybridization. The probes for chromosome were CEPX/Y and CEP18. The rates of sperm aneuploidy were compared in groups A, B and C. Results： Three thousands sperms were counted, and the hybridization rate was 97%. There was a significant difference in the average rates of aneuploid sperms in group A （6.72±2.64） %, n=500, group B （1.60±1.02） %, n=1000, and group C （4.03±1.50） %, n=1500 （F = 9.622, P 〈 0.01）. There were significant differences in average rates of aneuploid sperms between group A and group B, and group A and group C （P 〈 0.01）, but no significant difference between group B and C （P 〉 0.05）. Conclusion： In the oligo-asthenospermia patient group treated by ICSI, the embryo should be tested by preimplantation genetic diagnosis （PGD） before transfer, especially in the severe oligo-asthenospermia patients.

关 键 词：原位杂交 荧光不育 男(雄)性精子非整倍体染色体 

分 类 号：R714.55[医药卫生—妇产科学] R596.104[医药卫生—临床医学]