乌司他丁对幼兔未成熟心肌缺血再灌注损伤的保护作用  

Myocardial Protective Effects of Ulinastatin on Ischemia-Reperfusion Injury in Isolated Immature Rabbit Heart Models

作　　者：刘春广[1] 陈庆良[2] 王轩[2] 

机构地区：[1]天津医科大学,300070 [2]天津市胸科医院

出　　处：《天津医药》2009年第6期495-497,共3页Tianjin Medical Journal

基　　金：天津市科技发展计划项目(项目编号:08ZCKFSF03300)

摘　　要：目的:研究乌司他丁对幼兔离体心脏缺血再灌注损伤的保护作用。方法:建立幼兔离体心脏Langendorff模型,灌注停跳液使心脏停跳30min,用加入乌司他丁的K-H液复灌30min。于缺血前0min,复灌后10、20、30min记录心率(HR)、左室内压(LVP)、左室内压最大上升速率(LVdp/dt)、冠脉流量(CF);于缺血前1min,复灌后10、20、30min测定冠状静脉流出液中肌酸激酶(CK)、肌钙蛋白(cTnI)、肿瘤坏死因子(TNF-α)、白细胞介素6(IL-6)的浓度。结果:(1)乌司他丁组心脏复灌后HR、CF、LVdp/dtmax均升高(P<0.05或P<0.01);冠状静脉流出液CK、cTnI、TNF-α、IL-6浓度均低于对照组(P<0.01)。(2)对照组心肌细胞横纹模糊不清,可见多灶性细胞崩解,心肌纤维断裂,间质充血、出血并有中性粒细胞和淋巴细胞浸润;乌司他丁组心肌细胞胞浆嗜伊红均质浓染,可见局灶性细胞崩解,间质充血只有少量淋巴细胞浸润。结论:乌司他丁能明显减轻幼兔未成熟心肌缺血再灌注损伤。Objective：To investigate the myocardial protective effects of ulinastatin on ischemia-reperfusion injury in isolated immature rabbit heart models and the mechanisms thereof. Methods：Forty New Zealand white immature rabbits were used in the study to establish Langendorff heart models. The hearts were mounted on Langendorff apparatus and perfused with oxygenated K-H solution at 37℃ for 30 min after 30 min global myocardial ischemia.The heart rate （HR）, left ventricular pressure（LVP）, maximum rate of left ventricular pressure rise （LVdp/dtmax） and coronary flow （CF）were monitored at 0 min before ischemia and 10, 20, 30 min after reperfusion. The concentrations of creatine kinase （CK）, cardiac troponini （cTnI）,tumor necrosis factor （TNF）-α and interleukin -6 （IL-6） of coronary sinus effluent were detected at time points of 1 min before ischemia and 10,20 and 30 min after reperfusion. Results：The values of HR, CF and LVdp/dtmax cTnI were increased after reperfusion in ulinastatin group（P 〈 0.05or P 〈 0.01）. The concentrations of CK, cTnI, TNF-α and IL-6 were significantly lower in gulinastain group than that of control group （P 〈 0.01）. Conclusion： Ulinastain significantly reduced myocardial reperfusion injury in isolated immature rabbit heart models.

关 键 词：心肌再灌注损伤 胰蛋白酶抑制剂 心肌缺血 疾病模型 动物 兔 

分 类 号：R542.2[医药卫生—心血管疾病] R576[医药卫生—内科学]