骨形态发生蛋白4 mRNA在大鼠后纵韧带减压后的表达(英文)  

Expression of bone morphogenetic protein-4 mRNA in the cervical posterior longitudinal ligament after decompression

作　　者：蔡国栋[1] 司萌[1] 张辉[1] 王俊勤[1] 

机构地区：[1]泰山医学院附属医院骨科,山东省泰安市271000

出　　处：《中国组织工程研究与临床康复》2009年第24期4783-4786,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:有学者认为手术增加了颈椎不稳定的风险,后纵韧带的张力有可能改变体内生长因子表达。然而两者是否存在关系还没有定论。骨形态发生蛋白(bone morphogenetic protein,BMP)4是具有独立异位成骨作用的细胞因子之一。目的:观察颈椎后路减压对颈椎后纵韧带中骨形态发生蛋白4mRNA表达的影响。设计、时间及地点:随机对照动物实验,于2005-01/2006-12在泰山医学院完成。材料:清洁级成年SD大鼠48只,雌雄不拘,体质量270～350g。方法:将大鼠随机分为3组。手术组:行C3～6全椎板切除减压加椎旁肌切断手术;假手术组:仅作皮肤切口;空白组:不作任何处理为。于处理后1,2,4,8周分批处死动物,取其后纵韧带。主要观察指标:采用半定量反转录-聚合酶链反应方法检测组织骨形态发生蛋白4 mRNA表达水平。结果:空白组及假手术组未见明显骨形态发生蛋白4 mRNA的表达;手术组于手术后1周表达明显,持续到8周。结论:颈椎后路减压手术可以导致后纵韧带早期细胞骨形态发生蛋白4 mRNA表达上升,内源性的骨形态发生蛋白4可能导致手术后韧带骨化发展。BACKGROUND： It has been thought that posterior cervical decompression increases the risk of cervical instability and the stress on posterior longitudinal ligament may change growth factor expression in vivo. However, the association between BMP-4 expression and heterotopic bone formation has not been confirmed. Bone morphogenetic protein 4 （BMP-4） is one of factors that can lead to heterotopic bone formation alone. OBJECTIVE： To observe the effects of posterior cervical decompression on BMP-4 mRNA expression in the posterior longitudinal ligament. DESIGN, TIME AND SETTING： A randomized controlled animal experiment was performed in Taishan Medical College between January 2005 and December 2006. MATERIALS： A total of 48 adult Sprague Dawley rats of either gender and of clean grade, weighing 270 350 g, were included in this study. METHODS： All rats were randomly and evenly divided into 3 groups： experimental, sham-operated, and blank control. In the experimental group, C3-6 laminectomy for decompression was performed. In the sham-operated group： only skin incision was made. Rats in the blank control group received no any treatment. Four rats were allocated for each time point （1, 2, 4, and 8 weeks post-surgery） for harvesting posterior longitudinal ligament. MAIN OUTCOME MEASURES： Detection of BMP-4 mRNA expression in the cervical posterior longitudinal ligament by semi-quantitative reverse transcription-polymerase chain reaction. RESULTS： In the experimental group, obvious BMP-4 mRNA expression was found at 1-8 weeks post-surgery. While no BMP-4 mRNA expression was found at all times in the sham-operated and blank control groups. CONCLUSION： Posterior cervical decompression can increase BMP-4 mRNA expression in the posterior longitudinal ligament. Endogenous BMP-4 may contribute to Iigamentous ossification and development post-surgery.

关 键 词：骨形态发生蛋白质类 颈椎 组织构建 

分 类 号：R318[医药卫生—生物医学工程]