马缨杜鹃壮苗与生根培养基的筛选研究  

Selection of Medium for Budding and Rooting in Tissue Culture of Rhododendron delavayi

作　　者：何承忠[1] 许昌慧[1] 王立苍 江涛[3] 辛培尧[1] 

机构地区：[1]西南林学院资源学院,昆明650224 [2]曲靖市林业局,云南曲靖655000 [3]西南林学院学生工作处,昆明650224

出　　处：《湖北农业科学》2009年第5期1042-1044,1054,共4页Hubei Agricultural Sciences

基　　金：云南省教育厅基金项目(07C11314);云南省重点建设学科西南林学院森林培育学基金项目(500018)

摘　　要：以马缨杜鹃组织培养苗为试材,进行了壮苗和生根培养基配方的研究,为最终建立马缨杜鹃组织培养和快速繁殖技术体系奠定基础。结果表明,壮苗的最适培养基为添加蔗糖20 g.L-1和卡拉胶6 g.L-1的WPM,最适于生根的培养基为WPM+NAA 0.5 mg.L-1+IBA 1.00 mg.L-1+活性炭0.2%+蔗糖20 g.L-1,生根率达到85.0%。In order to establish the tissue culture and rapid propagation system of Rhododendron delavayi, the plantlets used as materials, experiment was conducted to select the optimal medium for budding and rooting in tissue culture of Rhododendron delavayi. The results showed that the optimal budding medium was WPM with sugar 20 g·L^-1 and earrageenan 6 g·L^-1, and the medium for rooting culture was WPM＋NAA 0.5 mg·L^-1＋IBA 1.00mg·L^-1＋AC 0.2%＋sugar 20g·L^-1,on which the rate of rooting was 85.0%.

关 键 词：马缨杜鹃 壮苗 生根培养 培养基 

分 类 号：S685.21[农业科学—观赏园艺] Q943.1[农业科学—园艺学]