白腐菌Coriolus versicolor的培养及产漆酶条件的研究  

Studies on Culture Conditions for Laccase Production from White-rot Fungus Coriolus versicolor

作　　者：吴香波[1] 谢益民[1] 冯晓静[1,2] 

机构地区：[1]山东轻工业学院造纸科学与技术教育部重点实验室,山东济南250353 [2]山东省环境保护科学研究设计院,山东济南250013

出　　处：《纤维素科学与技术》2009年第2期12-19,共8页Journal of Cellulose Science and Technology

基　　金：国家自然科学基金资助项目(30471365;20674046)

摘　　要：从液体和固体两种培养体系出发,探索不同培养条件对白腐菌分泌漆酶的影响。液体培养体系中,白腐菌的最佳生长和漆酶分泌条件是缓冲体系起始pH值为5.8、摇瓶转速为180r/min、装液量为100mL/500mL;在所有测试的碳源中,葡萄糖为最佳碳源,在二糖中以蔗糖为碳源,漆酶酶活相对较高,而多糖(如淀粉)不利于漆酶的生成。在所有测试的氮源中,硝酸铵和酒石酸铵组合为最佳氮源,氯化铵和尿素的效果次之,蛋白胨的效果最差。当碳源与氮源质量比(C/N)大于10∶1,即高碳低氮的培养条件有利于漆酶的生成。芳香化合物诱导剂ABTS浓度为0.5～1mmol/L时,对产漆酶的影响最明显,与限氮培养基中产酶相比可提高酶活5倍,约为230IU/mL;0.5mmol/L愈创木酚、1mmol/L阿魏酸和0.05mmol/L二甲基苯胺的添加可提高酶活约2～2.5倍;0.01mmol/L黎芦醇的添加可提高漆酶酶活约1.6倍。由于酪氨酸具有酚类化合物的特点,也可作为一种无毒的天然漆酶诱导剂。菌株漆酶活力在固体培养体系明显低于在液体培养体系。Firstly, the influence on the production of laccase by Coriolus versicolor in different fermentation conditions and culture systems were investigated. In the liquid culture system, the optimal fermentation condition for best fungus growth and laccase producing were as follows：buffering system ofpH 5.8, rotating speed 180 r/min, 100 mL culture medium per 500 mL flask. The glucose was the best carbon fountain in all the tested carbon fountains. The laccase activity was comparative high when saccharose was used as the carbon fountain in all the disaccharide. However, starch appeared minus effect on laccase production. The combination of tartaric ammonium and ammonium nitrate was found to be the best nitrogen fountain in all the tested nitrogen fountains. The effect of ammonium chloride and carbamide took second place. The effect of peptone was the worst. It was suitable to the production of laccase when the ratio of carbon and nitrogen is more than 10. The laccase activity could be improved to 230 IU/mL when the revulsant ABTS concentration is 0.5-1 mmol/L. The addition of 0.5 mmol/L guaiacol, 1 mmol/L ferulic acid and 0.05 mmol/L dimethylaniline could also improve the laecase activity obviously. The addition of 0.01 mmol/L veraryl alcohol could also improve the laccase activity for 1.6 times. Tyrosine could be a native revulsant of laccase because it has the characteristic of hydroxybenzene The laccase activity was less in solid conditions than in liquid system.

关 键 词：杂色云芝菌 漆酶 

分 类 号：Q55[生物学—生物化学]