不同抗凝剂对红细胞渗透脆性试验结果的影响  

Effect of different anticoagulant on red cell osmotic fragility test

作　　者：肖小慧[1] 魏寿忠[1] 黄素丹[1] 张捷[1] 

机构地区：[1]福建省宁德市医院检验科,352100

出　　处：《检验医学与临床》2009年第12期966-966,968,共2页Laboratory Medicine and Clinic

摘　　要：目的了解EDTA-K2、枸橼酸钠、肝素锂抗凝血对红细胞渗透脆性试验结果的影响。方法对40份分别用EDTA-K2、枸橼酸钠、肝素锂抗凝的血标本进行红细胞渗透脆性试验;剩余血液用0.9%NaCl洗涤3次后配成50%红细胞悬液再进行试验;同时进行不加抗凝剂的对照试验。结果EDTA-K2抗凝血标本及洗涤后的渗透脆性均明显高于对照组,枸橼酸钠、肝素锂抗凝血标本对渗透脆性有一定影响,经洗涤后与对照组没有明显的差别。结论可以用枸橼酸钠或肝素锂抗凝血标本经洗涤后进行红细胞渗透脆性试验,不能用EDTA-K2抗凝的标本。Objective To investigate the effect of anticoagulative blood samples with EDTA-K2 ,sodium citrate and lithium-heparin on red cell osmotic fragility test result. Methods 40 EDTA-K2, sodium citrate and lithium-heparin treated blood samples were tested for red cell osmotic fragility. The rest of blood was confected into 50% red cell suspensions after three times washing-up by 0.9% saline for red cell osmotic fragility test. At the same time, the blood samples for controlled trials were free from anticoagulant. Results The red cell osmotic fragility of EDTA-K2 treated blood was obviously higher than the controlled trials, even after washing-up. To some extent, sodium citrate and lithium-heparin treated blood could affect the results. However, it showed there was no significant difference compared with the control group after washing-up. Conclusion Sodium citrate treated and lithium-heparin treated blood samples can be used for red cell osmotic fragility test after washing-up by saline but not EDTA-K2 treated blood.

关 键 词：红细胞渗透脆性试验 EDTA—K2 枸橼酸钠 肝素锂 

分 类 号：R446.11[医药卫生—诊断学]