Determination of amitriptyline and nortriptyline in human liver microsomes with reversed-phase HPLC in vitro  

Determination of amitriptyline and nortriptyline in human liver microsomes with reversed phase HPLC in vitro 1

作　　者：舒焱[1] 朱荣华[1] 许振华[1] 周宏灏[1] 

机构地区：[1]湖南医科大学遗传药理学研究所

出　　处：《中国药理学报》1998年第4期343-346,共4页Acta Pharmacologica Sinica

摘　　要：ＤｅｔｅｒｍｉｎａｔｉｏｎｏｆａｍｉｔｒｉｐｔｙｌｉｎｅａｎｄｎｏｒｔｒｉｐｔｙｌｉｎｅｉｎｈｕｍａｎｌｉｖｅｒｍｉｃｒｏｓｏｍｅｓｗｉｔｈｒｅｖｅｒｓｅｄｐｈａｓｅＨＰＬＣｉｎｖｉｔｒｏ１ＳＨＵＹａｎ，ＺＨＵＲｏｎｇＨｕａ２，ＸＵＺｈｅｎＨｕ...AIM: To develop a method for simultaneous determinations of amitriptyline (Ami) and its metabolite nortriptyline (Nor) in human liver microsomes. METHODS: An incubation buffer containing microsomes, NADPH generating system, and Ami, after termination of enzyme reaction and desipramine (Des) as internal standard (IS), was extracted with diethy ether and separated on a reversed phase ODS column. Detection was achieved at 242 nm by ultraviolet detector. RESULTS: No potential interfering peaks were found. Ami and Nor gave rapid elution and baseline resolution. The linear curves of both analyses ranged 0 02-10 nmol and the limit of detection was 0 01 nmol. The recovery (94 %-101 %) had good precision with relative s of <8 3 % . CONCLUSION: This method is rapid, sensitive, and simple for studying the metabolism of Ami and Nor.

关 键 词：阿米替林 去甲替林 高压液相色谱法 肝微粒体 

分 类 号：R971.4[医药卫生—药品] R927.2[医药卫生—药学]