黄芩素对人子宫颈癌HeLa细胞生长的抑制作用  

Inhibitory effect of baicalein on human cervix cancer HeLa cells

作　　者：童旭辉[1,2] 董淑英[1] 陶亮[2] 

机构地区：[1]蚌埠医学院药理学教研室,安徽蚌埠233030 [2]中山大学中山医学院药理学教研室,广东广州510080

出　　处：《蚌埠医学院学报》2009年第6期468-470,共3页Journal of Bengbu Medical College

摘　　要：目的:探讨黄芩素对人子宫颈癌HeLa细胞生长的抑制作用及可能机制。方法:采用磺酰罗丹明B(SRB)法检测黄芩素对HeLa细胞生长的抑制作用;Hoechst33258荧光染色法观察黄芩素对HeLa细胞凋亡的诱导作用;比色法测定黄芩素对HeLa细胞内caspase-3活性的影响。结果:不同浓度黄芩素作用24、36、48h后均可抑制HeLa细胞生长(P<0.05～P<0.01);黄芩素作用24h后,HeLa细胞凋亡率增加(P<0.01),细胞内caspase-3活性增强(P<0.05～P<0.01)。结论:黄芩素能抑制HeLa细胞生长,其机制可能与诱导细胞凋亡及增强caspase-3活性有关。Objective：To investigate the role of baiealein in inhibiting the HeLa cells of human cervix cancer and the possible mechanism. Methods ： Sulforhodamine B （ SRB ） assay was used to determine the inhibitory effect of baicalein on HeLa cell growth ; cellular apoptosis was detected by Hoechst 33258 fluorescent staining;caspase-3 activity was assayed by colorimetric method. Results： After treated with different concentration of haicalein for 24,36,48 hour, HeLa cell growth were significantly inhibited（ P 〈 0.05 to P 〈 0.01 ） ; the apoptosis rate of HeLa cells（ P 〈 0.01 ） and the activity of caspase-3 （ P 〈 0.05 to P 〈 0.01 ） were increased when HeLa cells were treated with baicalein for 24 hours. Conclusions： Baicalein can inhibit the growth of HeLa cells, and the inhibitory effect probably results from the increased cell apoptosis and caspase-3 activity.

关 键 词：子宫颈肿瘤 HELA细胞 黄芩素 细胞凋亡 CASPASE-3 

分 类 号：R273.733[医药卫生—中西医结合] R737.33[医药卫生—中医肿瘤科]