玉米抗甘蔗花叶病毒SCAR分子标记开发  

Development of SCAR Markers for Sugarcane Mosaic Virus Resistance in Maize

作　　者：吕香玲[1,2] 王邦太[2] 史利玉[2] 石红良[2] 谢传晓[2] 李新海[2] 张世煌[2] 

机构地区：[1]沈阳农业大学农学院,沈阳110161 [2]中国农业科学院作物科学研究所,农作物基因资源与基因改良国家重大科学工程,北京100081

出　　处：《中国农业科学》2009年第6期1980-1987,共8页Scientia Agricultura Sinica

基　　金：国家高技术研究发展计划(“863”项目)(2006AA100103、2007AA10Z172)

摘　　要：【目的】玉米矮花叶病(由甘蔗花叶病毒引起)是中国和欧洲玉米产区的重要病害,开展分子标记辅助育种可以明显提高抗病育种效率。【方法】本文采用改进的BSA法(bulked segregant analysis,BSA)构建玉米抗感自交系DNA池,结合AFLP技术筛选多态性标记,转化后获得实用性强的SCAR标记,并借助100份自交系抗性鉴定结果对SCAR标记进行相关验证。【结果】获得了2个多态性稳定的AFLP标记P66M38-220和P55M51-240,其中将P66M38-220转化为SCAR112标记,此标记与甘蔗花叶病毒抗性高度相关。【结论】改良BSA法是一种发掘性状基因紧密连锁标记的有效方法;开发的SCAR112标记可以用于玉米抗甘蔗花叶病毒的分子标记辅助选择。[Objective ] Maize dwarf mosaic disease caused by sugarcane mosaic virus （SCMV） is one of the important virus diseases in China and European countries. Marker development and markers-assisted selection （MAS） is an efficient way for disease-resistant breeding. [ Method] Resistant and susceptible DNA bulks were composed by using DNAs of 10 resistant and 12 susceptible inbred lines, respectively. Polymorphic AFLP markers were screened between two bulks and then transformed into SCAR （sequence characterized amplified region） markers. These SCAR markers associated with SCMV resistance was analyzed with disease incidence of 100 inbred lines. [Result] Two polymorphic AFLP markers, P66M38-220 and P55M51-240, were identified, and P66M38-220 was transformed into SCAR112, which was validated to be highly associated with SCMV resistance. [Conclusion] Improved BSA method is an efficient way for developing markers tightly linked with target traits. The SCAR112 developed in this study could be used for MAS of sugarcane mosaic virus resistance in maize.

关 键 词：玉米 甘蔗花叶病毒 改良BSA法 AFLP标记 SCAR标记 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]