富贵竹中杆状DNA病毒湖北分离物的分子鉴定  

Molecular Identification of Badnavirus in Dracaena sanderiana from Hubei of China

作　　者：陈秀[1] 阮小蕾[1] 赵芹[1] 李华平[1] 

机构地区：[1]华南农业大学植物病毒研究室,广州510642

出　　处：《中国农业科学》2009年第6期2002-2009,共8页Scientia Agricultura Sinica

基　　金：国家自然科学基金(30671358)

摘　　要：【目的】证明湖北发病富贵竹上是否存在杆状DNA病毒属(Badnavirus)病毒,分析来自富贵竹及其它作物Badnavirus病毒不同分离物间的分子差异。【方法】通过分段克隆测序的方法获得湖北富贵竹中Badnavirus病毒基因组全序列,利用BLAST工具及其它生物学软件进行序列分析。【结果】富贵竹Badnavirus病毒湖北分离物基因组为环状结构,全长7522bp;基因组正链包含7个ORFs,推测其编码蛋白的分子量分别为17.58、14.93、214.77、11.86、11.31、16.12和11.00kD。获得的基因组与福建富贵竹斑驳病毒(Dracaenamottlevirus,DrMV)大小仅相差9个核苷酸,两者基因组核苷酸序列一致率为99.7%,ORFs1～3编码氨基酸序列的一致率分别为99.3%、100%、99.2%,而与其它已报道的14种Badnavirus病毒分离物间的核苷酸全序列一致率为32.0%～44.0%。【结论】叶片表现褪绿斑驳等症状的湖北富贵竹中存在Badnavirus病毒,该病毒与DrMV为同种病毒,已报道的富贵竹中Badnavirus病毒分离物间分子差异非常小,这与已报道的其它作物中Badnavirus病毒不同分离物间存在非常大的分子差异不同。[Objective] In order to identify badnavirus in Dracaena sanderiana with typical mottle symptom from Hubei of China, and to compare the genome molecular variation between isolates ofDracaena sanderiana badnaviruses, the complete genome sequence of Dracaena sanderiana badnavirus Hubei isolate was obtained. [Method] The complete genome sequence was got through subsection cloning and sequencing, and the sequences were analyzed with BLAST and other softwares. [Result] The complete circular genome nucleotide sequence comprised 7 522bp, which contained 7 open reading frames （ORFs） on the plus-strand that potentially encoded proteins of 17.58 kD, 14.93 kD, 214.77 kD, 11.86 kD, 11.31 kD, 16.12 kD and 11.00 kD. The genome organization was highly similar with the newly reported badnavirus Dracaena mottle virus （DrMV） . The size of Hubei isolate was 9 nucleotides shorter than DrMV. They shared 99.7% identities over the entire nucleotide sequences, and 99.3%, 100%, 99.2% over the presumed amino acid sequences of ORFs 1-3, respectively. [Conclusion] The genome organization and the comparison analysis of sequences between the genomes of badnaviruses revealed that Dracaena sanderiana with typical mottle symptom from Hubei of China contained badnavims, and Dracaena sanderiana badnavirus Hubei isolate and DrMV belonged to the same species, and the genome molecular variation between isolates ofDracaena sanderiana badnavirus was very small, which was much different from previous results that significant molecular variation existed in badnavirus isolates from different plants.

关 键 词：富贵竹 杆状DNA病毒 分子鉴定 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]