猪精子黏合分子1(SPAM1)基因在白色杜洛克×二花脸F_2资源群体中的遗传变异及其与母猪产仔数的关联性  

Genetic Variation of Porcine Sperm Adhesion Molecule 1 (SPAM1) Gene and Its Association with Litter Size in a White Duroc×Erhualian Resource Population

作　　者：吴延博[1] 陈从英[1] 张志燕[1] 郭源梅[1] 高军[1] 

机构地区：[1]江西农业大学动物生物技术国家重点实验室培育基地,南昌330045

出　　处：《中国农业科学》2009年第6期2111-2117,共7页Scientia Agricultura Sinica

基　　金：国家"973"计划(2006CB102103);江西省自然科学基金项目(0630052;2007GZN0360)

摘　　要：【目的】猪精子黏合分子1(SPAM1)基因在精卵结合过程中发挥重要作用,是影响产仔数的重要候选基因。本试验分析SPAM1基因的遗传变异及其与母猪产仔数的相关性。【方法】以305头白色杜洛克×二花脸资源群体F2母猪为材料,记录连续3胎的产仔性状,利用PCR-RFLP检测intron1 g298C>T、intron1 g357C>T和intron3 g380C>T共3个SNP位点在F2群体中的多态性,构建单倍型,分析各SNP基因型和单倍型与母猪产仔数、产活仔数、产死胎数的相关性。【结果】intron1 g298C>T位点CC型母猪的总产仔数显著低于CT、TT型母猪(P<0.05),CT基因型母猪产死胎数显著多于TT型(P<0.05)。intron1 g357C>T位点CT基因型母猪产死胎数显著多于CC、TT基因型(P<0.05)。intron3 g380C>T位点CC基因型母猪产活仔数显著低于CT型,产死胎数显著高于CT型(P<0.05)。在6种单倍型中,TTT单倍型母猪的总产仔数和产活仔数明显高于其它5种(P<0.05);TCT、TTC单倍型母猪产死胎数显著低于CTC、TTT和TCC(P<0.05)。【结论】SPAM1基因与母猪产仔数存在显著相关性。[Objective] Porcine sperm adhesion molecule 1 （SPAM1） gene plays an important role in fertilization. It is an important candidate gene for porcine litter size. In this study, identification of genetic variation of SPAM1 gene and association study with litter size were carded out. [Method] Detection ofpolymorphisms ofintronl g298 C〉T, intronl g357 C〉T and intron3 g380 C〉T and construction of haplotypes were carried out in 305 F2 sows from a white Duroc×Erhualian resource population, whose litter size was recorded in detail in 3 subsequent parities. Association study of genotypes of each SNP and haplotypes with total number born （TNB）, number born alive （NBA） and number stillborn （NSB） was also performed. [Result] The results showed that, compared to individuals of CT and TT at SNP intronl g298 C〉T, individuals of CC had significantly less TNB and NBA （P〈0.05）. Sows of CT had significantly higher number of NSB than sows ofTT （P〈0.05）. For SNP intronl g357 C〉T, the CT sows produced significantly more NSB compared to CC and TT individuals. And for SNP intron3 g380 C〉T, CC individuals had significantly less NBA and more NSB than CT individuals. Six haplotypes were constructed in this study. Haplotype TTT showed significantly higher TNB and NBA than the other 5 haplotypes. Individuals of TCT and TTC had significantly less NSB than individuals of CTC, TTT and TCC （P〈0.05）. [Conclusion] SPAM1 gene has significant association with porcine litter size.

关 键 词：SPAM1基因 SNPs 单倍型 产仔数 猪 

分 类 号：S828[农业科学—畜牧学]