复方中药提取物对REV诱发的免疫抑制鸡的免疫增强作用  

Immune Enhancement Effect of Mixed Herbal Extracts in Chicks Immnunosuppressed by Reticuloendotheliosis Virus Infection

作　　者：柳风祥[1] 崔治中[1] 郭慧君[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《中国农业科学》2009年第6期2164-2171,共8页Scientia Agricultura Sinica

基　　金：国家自然科学基金重点项目(30330450)

摘　　要：【目的】探讨复方中药提取物对REV诱发的免疫抑制鸡的免疫增强作用,建立测试某些中药制剂免疫增强作用的实验模型。【方法】分别在临床健康鸡和在1日龄感染网状内皮增生病病毒(REV)诱发了免疫抑制状态的鸡测试了中药制剂对鸡的免疫增强作用。【结果】随饮水饲喂黄芪、党参、淫羊藿和甘草复方中药浸出物,在健康鸡对鸡新城疫病毒(NDV)和H5亚型禽流感病毒(H5-AIV)灭活疫苗免疫后的血凝抑制(HI)抗体滴度没有明显影响。但是在REV诱发了免疫抑制的鸡,该中药浸出物可显著提高对NDV和H5-AIV的HI抗体滴度(P<0.01),虽然仍显著低于未经REV感染的对照鸡。【结论】REV诱发的免疫抑制状态下的鸡,可作为测试某些中药制剂免疫增强作用的一种实验模型。[Objective] To research immune enhancement effect of mixed herbal extracrs in chicks immunosuppressed by reticuloendotheliosis virus infection and establish an experiment model for determination of immune enhancing effects of some herbs [Method]Immune enhancement of herbal extracts was determined in clinical health chicks and chicks with immunosuppression induced by reticuloendotheliosis virus （REV） infection. [ Result] Hemagglutination inhibition （HI） antibody titers against Newcastle disease virus （NDV） and H5 avian influenza virus （H5-AIV） after vaccination were not influenced by supplement with the extract of mixed herbs, Radix Astragali, Radix Codonopis, Herba Epimedii and Radix Glycyrrizae in drinking water in clinical health chicks. In chicks immunosuppressed by reticuloendotheliosis virus （REV） infection, however, supplement of the same herbal extract in drinking water significantly increased HI antibody titers to NDV and H5-AIV when compared to the immunosuppressed control group （P〈0.01）. [Conclusion] It is concluded that chicks with REV-induced immunosuppression could be used as an experiment model for determination of immune enhancing effects of some herbs.

关 键 词：中药提取物 免疫抑制 REV 体液免疫 

分 类 号：S858.31[农业科学—临床兽医学] TQ658[农业科学—兽医学]