连翘酯苷对小鼠的急性毒性及体内诱生IFN-α的研究  

The Acute Toxicity of Forsythiaside and the Effects on Inducing IFN-α in Mice

作　　者：毛东有[1] 张中文[1] 杨明[1] 王惠川[1] 吴国娟[1] 

机构地区：[1]北京农学院动物科学技术系,北京102206

出　　处：《动物医学进展》2009年第6期15-17,共3页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(30771625);北京市自然科学基金项目;北京市属高校人才强教计划资助项目

摘　　要：测定连翘酯苷对小鼠腹腔注射急性毒性试验的半数致死量(LD50),检测连翘酯苷体内诱生干扰素的效果。腹腔注射连翘酯苷,用改良寇氏法计算半数致死量。眼部采血,ELISA方法测定血清中IFN-α。测得连翘酯苷对小鼠腹腔注射急性毒性的LD50为1 976.5 mg/kg,LD50的95%可信限为1 863.7 mg/kg^2 096.1 mg/kg。连翘酯苷诱生干扰素在48 h达到最高峰845.8 pg/mL±150.92 pg/mL(P<0.01)。表明连翘酯苷为低毒化合物,体内能够诱生IFN-α。The LD50 of acute toxicity test in mice was measured by way of intraperitoneal injection, the effect of forsythiaside inducing IFN-α was detected in vivo. Forsythiaside was injected in abdominal, the LD50 was calculated using the improved Karbel＇s method. The blood was collected from eyeball,and the IFN-α in the blood serum was detected using ELISA kit. The （LDso） forsythiaside given to mice by intraperitoneal injection was 1 976.5 mg/kg BW. The 95% confidence interval were 1 863.7 mg/kg -2 096.1 mg/kg BW. Forsythiaside could induce mouse to produce IFN-α and reach the peak 845.8 pg/mL＋ 150. 92 pg/mL （P 〈0. 01）at 48 h after injection. The results confirmed that forsythiaside is a low poison chemical compound and can induce IFN-α in vivo.

关 键 词：连翘酯苷 急性毒性 Α干扰素 小鼠 

分 类 号：S853.74[农业科学—临床兽医学]