光盐交互胁迫下台湾沟叶结缕草生长、叶绿体膜脂过氧化和ATPase动态变化研究  

Dynamic Changes of Growth,Chloroplast Lipid Peroxidation and ATPase Activity of Taiwan Zoysia matrella under Light and Salt Stress

作　　者：王加真[1] 夏更寿[1] 李建龙[2] 

机构地区：[1]丽水学院园林系,浙江丽水323000 [2]南京大学生命科学学院,江苏南京210093

出　　处：《江西农业大学学报》2009年第3期487-493,共7页Acta Agriculturae Universitatis Jiangxiensis

基　　金：浙江省农业科技攻关资助项目(2005C22079)

摘　　要：台湾沟叶结缕草接受强辐射、极度遮荫胁迫后,形态上表现出适阳型、适阴型两种生态型。继续实施弱光、弱光+盐胁迫处理,在0.5 mol/L NaCl溶液水培通气24 d后,所有处理下叶绿体可溶性蛋白、叶绿体MDA表现持续上升的趋势,与弱光相比,光盐胁迫后上升幅度更大;叶绿体Na+-K+-ATPase活力在弱光下无大的变化,适阳型在光盐胁迫下持续上升,而适阴型在16 d为最高,24 d下降。阳生型在弱光、光盐胁迫下叶绿体Ca2+-ATPase活力持续上升;阴生型在弱光下叶绿体Ca2+-ATPase活力在16 d时最高,之后下降;阴生型在弱光、光盐胁迫下叶绿体Ca2+-ATPase活力8 d时最高,之后下降。这表明:适阴型与适阳型相比,抗盐性明显下降,生理上表现出叶绿体膜脂过氧化程度加剧,Na+、K+跨膜转运、渗透调节功能下降,细胞膜稳定性下降;形态上表现出萎蔫的症状。After the treatment of strong radiation and extreme shade, different light types of Taiwan Zoysia matreUa were treated with the low- light, low- light ＋ 0.5 mol/L NaC1 stress. 24 days later, the sun -type growth tended to that of the shade - type, and the original shade - type turfgrasses suffered seriously, resulting in partly wilting. Under all treatments, chloroplast soluble protein, chloroplast MDA continued to rise, compared with low light, light and salt stress resulted in even greater rise. Activity of chloroplast Na ^＋ - K^＋ - AT- Pase under low -light had no major changes, but the sun -type under light and salt stress continued to rise, the shade - type under light and salt stress reached the maximum on the 16th day,but began to drop on the 24th day. Ca^2＋ -ATPase activity of the suntype under light, light and salt stress continued to rise;Ca^2＋ - ATPase acticity of the shade -type under low -light showed the highest on the 16th day, then declined. Under low-light ＋ salt stress,Ca^2＋ -ATPase activity reached the maximum on the 8th day, then declined. These results show that： the sun - type has a worse salt - tolerance than the shade - type, the physical performance is aggravated chloroplast membrane lipid peroxidation, declined regulatory function of Na^＋ , K^＋ transmembrane and dropped membrane stability.

关 键 词：台湾沟叶结缕草 光盐胁迫 叶绿体 丙二醛 渗透调节功能 跨膜转运 

分 类 号：S688.401[农业科学—观赏园艺]