抗生素溶杆菌对魔芋软腐病和根际微生物多样性的影响  

Effects of Lysobacter antibioticus on Soft Rot Disease and the Diversity of Konjac Rhizosphere Microbial Community

作　　者：姬广海[1] 吴亚鹏[1] 白学慧[1] 卢俊 董坤 

机构地区：[1]云南农业大学植物保护学院,云南昆明650201 [2]云南省富源魔芋研究所,云南富源655400

出　　处：《江西农业大学学报》2009年第3期499-503,544,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金(30760139);农业部农业行业科研专项经费(nyhyzx07-056)资助

摘　　要：研究施入抗生素溶杆菌菌剂对魔芋软腐病的控制作用,同时采用Biolog方法分析该生防细菌对魔芋根际微生物多样性的影响。田间试验结果显示:施入生防细菌后,抗生素溶杆菌能有效控制魔芋软腐病,还有增产作用,防效可达79.16%,增产14.30%,显著高于水合霉素和对照;还可不同程度地提高魔芋根际微生物Shan-non多样性、均匀度指数和每孔颜色平均变化率(AWCD);并且魔芋根际微生物对碳源的利用情况也发生了改变。与对照相比,施入生防菌剂的处理,对聚合物、胺类、酚类化合物的碳源利用率得以提高,而对糖类、羧酸类、氨基酸类的碳源利用率降低。综合分析认为,施入生防细菌菌剂对魔芋根际微生物群落多样性无明显的负面影响。The experiments were carried out to investigate the effects of L. antibioticus biocontrol agent on suppressing soft rot of konjac and metabolic functional diversity of konjac rhizosphere microbial community by biolog ECO microplates. The results showed that bio - control agent could suppress the disease and increased yield effectively,bio - control efficacy amount to 79.16% and crop yield increased by 14.30%. In addition to Shannon diversity, Shannon evenness and the average well color development （AWCD） were influenced by bio - control agent. The ability of utilizing carbon source of konjac rhizosphere microbe varied in different treatments. Compared with water control（CK） ,L. antibioticus treatment raised the ability of utilizing polymers, amines and phenolic compounds, but reduced the ability of utilizing carbohydrates, carboxylic acids, amino acids. In conclusion, it is suggested that effects of L. antibioticus agents on the diversity of microbial community diversity of Konjac rhizosphere were not significantly negative.

关 键 词：Biolog-Eco方法 魔芋细菌性软腐病 抗生素溶杆菌 微生物群落 多样性 

分 类 号：S476.8[农业科学—农业昆虫与害虫防治]