五指山小型猪PMSA6基因cDNA克隆及生物信息学分析  

Cloning and Sequence Analysis of PMSA6 cDNA from Wuzhishan Miniature Pig

作　　者：刘涛[1] 申明霞[1] 杜丽[1] 程立生[1] 王凤阳[1] 成鹰[1] 李治深[1] 

机构地区：[1]海南大学农学院海口市动物基因工程重点实验室,海南海口570228

出　　处：《海南大学学报（自然科学版）》2009年第2期134-138,143,共6页Natural Science Journal of Hainan University

基　　金：国家自然科学基金资助项目(30560021);海南省自然科学基金资助项目(30509);海南省教育厅高校科研资助项目(Hj200804)

摘　　要：为了对海南五指山小型猪蛋白酶体亚基α型6(proteasome subunit alpha type 6,PMSA6)cDNA基因进行克隆和生物信息学分析,笔者以构建的五指山小型猪外周血白细胞cDNA文库为材料,采用菌落PCR方法,克隆得到PMSA6全长cDNA,并向GenBank递交了该序列(登录号:FJ358606).同时运用生物信息学软件对该基因核苷酸序列进行了分析,并预测了其编码蛋白的理化性质及二级结构等.生物信息学分析表明:该cDNA全长1029 bp,5′非翻译区长96 bp,3′非翻译区长192 bp,含有一个741 bp完整的开放阅读框,编码246个氨基酸.该蛋白的分子量为37.680 kD,等电点为8.76.进一步比对分析发现,五指山小型猪PMSA6基因的核酸序列及其氨基酸序列与人、牛等哺乳动物具有很高的相似性.本研究成功克隆了海南五指山小型猪PM-SA6基因cDNA,并进行了相关生物信息学分析,为进一步研究PMSA6在动物体内的作用机理奠定了基础.The research aimed to clone full-length cDNA of PMSA6 （proteasome subunit alpha type 6 ）gene of Wuzhishan Miniature Pig and analysis of this gene. A full-length PMSA6 gene eDNA was cloned from the eDNA library of the peripheral blood leucocytes in Wuzhishan Miniature Pig by Colony PCR. The cloned PMSA6 eDNA sequence had been sent to Gen-Bank and got a accession number： FJ358606. The bioinformatic analysis results showed that the PMSA6 cDNA sequence was 1029 bp length, containing an open reading frame of 741 bp, flanked by 96 bp 5＇UTR and 192 bp 3＇UTR, encoding 246 amino acids. Relative molecular weight was 37. 680 kD, pI was 8.76. Further analysis results indicated that the expression sequence of PMSA6 and the protein encoded were highly homologous to that of some mammals reported. This indicated that the Proteasome was evolutionary conservatively in animal kingdom, and the structure and function of PMSA6 gene was highly conservative.

关 键 词：五指山小型猪 CDNA文库 蛋白酶体亚基α型6 克隆 生物信息学分析 

分 类 号：Q78[生物学—分子生物学]