心肌纤维化大鼠Intermedin_(1-53)及其受体的变化  

The Expression of Intermedin_(1-53) and IMD_(1-53) Receptors in Hearts of Myocardial Fibrosis Rats

作　　者：薛立华[1] 梁颖[1] 闫琳[2] 宋君秋[3] 齐永芬[3] 贾月霞[1] 

机构地区：[1]宁夏医科大学基础学院病理生理学教研室,银川750004 [2]宁夏医科大学基础学院药理学教研室,银川750004 [3]北京大学医学部生理与病理生理学系,北京100083

出　　处：《宁夏医科大学学报》2009年第3期281-284,F0002,共5页Journal of Ningxia Medical University

基　　金：宁夏高等学校科学研究项目(宁高教[2006]237号)

摘　　要：目的在大鼠心肌梗死致心肌纤维化模型上观察Intermedin1-53及其受体的变化。方法通过结扎左冠状动脉前降支制备大鼠心肌梗死模型,4周后检测心功能,梗死区心肌组织的Ⅰ、Ⅲ型胶原蛋白表达,IMD1-53和心室肌降钙素受体样受体(CL)、受体活性修饰蛋白(RAMP)1/2/3的mRNA表达。结果与假手术组比较模型组大鼠的心功能明显减低,检测梗死区心肌组织的Ⅰ、Ⅲ胶原蛋白表达增加,心室肌IMD1-53、CL、RAMP1/2/3的mRNA水平分别上调75%(P<0.01)、57%(P<0.05)、61%(P<0.01)、48%(P<0.05)和80%(P<0.05)。结论心肌纤维化大鼠的心肌IMD1-53及其受体明显上调,其变化可能参与心肌纤维化的发病过程。Objective To observe the gene expression of IMD1-53 and IMD1-53 receptors in the myocardial fibrosis rats reduced by myocardial infarction. Methods Rats with Acute myocardial infarction （AMI） were induced by left anterior descending coronary branch ligation. The cardiac function was measured The protein levels of type Ⅰ and Ⅲ collagen were assayed by Western blotting and the mRNA levels of IMD1-53, CL, RAMP1,2, 3 were determined by semi - quantitative RT - PCR in the infracted hearts after 4 weeks of operation. Results Compared with the sham -operated group, cardiac function significantly decreased, protein levels of type Ⅰ and Ⅲ collagen significantly increased and the mRNA levels of CL, RAMP1, RAMP2 and RAMP3 significantly elevated by 75% （P〈0.01）, 57% （P〈0.05）, 61% （P〈0.01）, 48% （P〈0.05） and 80% （P 〈 0.05） respectively in myocardial fibrosis rat. Conclusion The results suggest that IMD1-53 and IMD1-53 receptors system is significantly up - regulated in myocardial fibrosis and such change maybe play a part in the procession of myocardial fibrosis.

关 键 词：Intermedin1-53 心肌纤维化 降钙素受体样受体 受体活性修饰蛋白 

分 类 号：R542.22[医药卫生—心血管疾病]