DEX和rhBMP2对体外培养人牙髓细胞碱性磷酸酶活性的影响  

Effects of DEX and rhBMP2 on alkaline phosphatase activity of human dental pulp cells in vitro

作　　者：张慧宇[1] 向学熔[1] 范小平[2] 

机构地区：[1]重庆医科大学附属口腔医院牙周黏膜科,重庆400015 [2]重庆医科大学附属口腔医院正畸科,重庆400015

出　　处：《第三军医大学学报》2009年第13期1264-1267,共4页Journal of Third Military Medical University

基　　金：重庆市卫生局科研基金(06-2-201)~~

摘　　要：目的探讨不同浓度地塞米松(Dexamethasone,DEX)、重组人骨形态发生蛋白2(recombinant human bone morphogenetic protein 2,rhBMP2)及两者联合应用对体外培养的人牙髓细胞(human dental pulp cells,HDPCs)碱性磷酸酶(Alkaline Phosphatase,ALP)活性的影响。方法组织块法培养HDPCs并进行鉴定;采用酶动力学的方法,观察DEX、rhBMP2及二者联合应用对HDPCs的ALP活性的影响。结果单独应用DEX时ALP活性明显增高,且在生理浓度范围内当浓度为0.01nmol/ml时,对HDPCs的ALP活性达到最大的刺激作用,rhBMP2对HDPCs的ALP活性呈浓度依赖性增强;当联合应用DEX和rhBMP2,ALP活性比单独应用相应浓度的rhBMP2明显增高。结论DEX、rhBMP2对HDPCs的ALP活性均有增强作用,同时二者对HDPCs的ALP活性有显著的功能放大性协同增强作用。Objective To study the effects of dexamethasone （DEX）, recombinant bone morphogenetic protein-2 （rhBMP2）, and combined application of rhBMP2 and DEX on alkaline phosphatase （ALP） activity of human dental pulp ceils （HDPCs） in vitro. Methods HDPCs were cultured by tissue block method and identified. Effects of DEX, rhBMP2, and combined application of both on ALP activity of HDPCs were determined by a modified enzyme dynamical method. Results DEX could enhance ALP activity, reaching the peak value at the concentration of 0.01 nmol/ml, rhBMP2 could enhance ALP activity in a dose-dependent manner. ALP activity was significantly higher under the condition of combined application of DEX and rhBMP2 than single application of DEX or rhBMP2 only. Conclusion Both DEX and rhBMP2 can enhance ALP activity of HDPCs. However, combined application of DEX and rhBMP2 can greatly enhance ALP activity of HDPCs.

关 键 词：地塞米松 重组人骨形态发生蛋白2 人牙髓细胞 碱性磷酸酶 

分 类 号：R345[医药卫生—基础医学] R446.19