绒山羊Scp3基因的克隆及睾丸中第一轮减数分裂的发生  

Cloning of Cashmere Goat Scp3 Gene and Determining the First Round Meiosis in the Testes

作　　者：侯越[1] 吴应积[1] 罗奋华[1] 苏慧敏[1] 张学明[1] 张岩[1] 白音巴图[1] 刘陶迪[1] 

机构地区：[1]内蒙古大学哺乳动物生殖生物学及生物技术教育部重点实验室,内蒙古呼和浩特010021

出　　处：《Zoological Research》2009年第3期341-344,共4页动物学研究（英文）

基　　金：国家高科技项目(863项目编号:2005AA206110)子课题;内蒙古自治区自然科学基金(200408020402)

摘　　要：联会复合体蛋白3(synaptonemal complex protein 3,Scp3)是雄性生殖细胞减数分裂中联会复合体形成必需的组成部分,在哺乳动物生殖细胞减数分裂中具有重要功能。通过RT-PCR扩增和分子克隆的方法首次克隆到了绒山羊Scp3基因的编码区片段。测序结果表明,绒山羊与牛的Scp3基因编码序列同源率达到98%。根据测得的DNA序列,设计引物,用RT-PCR方法分析测定了青春前期绒山羊不同个体中睾丸组织的Scp3的转录表达。结果显示,雄性绒山羊青春前期睾丸发育存在个体差异,已分析的样品第一轮减数分裂发生的时间普遍为73天之后。这一结果为绒山羊的睾丸发育和精子发生过程的相关研究打下了基础。The synaptonemal complex protein 3 （Sep3） plays a crucial role in synaptonemal complex formation and male germ cells meiosis. Until now, no studies on goat Scp3 have been reported. In present study, we cloned the Cashmere goat Scp3 cDNA using RT-PCR and molecular cloning techniques. The Scp3 sequence of Cashmere goat showed 98% identity with that of bovine. Primers were designed according to the sequence we got and RT-PCR was performed to analyze the expression of Scp3 in prepubertal Cashmere goat testes. The results showed that the testes development in prepubertal goat varies in different animals, and the first round of meiosis in analyzed Cashmere goat testes occurs mostly after 73 days after birth.

关 键 词：绒山羊 联会复合体蛋白3 克隆 睾丸发育 减数分裂 

分 类 号：Q78[生物学—分子生物学] Q953